Zinc Alpha 2 Glycoprotein Recombinant monoclonal antibody

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• Zinc Alpha 2 Glycoprotein Recombinant monoclonal antibody (86781-3-RR)

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  WB analysis of human plasma using 86781-3-RR

  human plasma were subjected to SDS PAGE followed by western blot with 86781-3-RR (AZGP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 86781-3-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86781-3-RR (Zinc Alpha 2 Glycoprotein antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 86781-3-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86781-3-RR (Zinc Alpha 2 Glycoprotein antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 86781-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86781-3-RR against Human Zinc Alpha 2 Glycoprotein were performed. The affinity constant is 63.7 pM.

Zinc Alpha 2 Glycoprotein Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 86781-3-RR

Clone No.251605B9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

AZGP1, Zinc-alpha-2-glycoprotein, ZA2G, ZAG, Zn alpha 2 glycoprotein

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Tested Applications

• Positive WB detected in: human plasma
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86781-3-RR targets Zinc Alpha 2 Glycoprotein in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg6736

  Product name: Recombinant Human Zinc Alpha 2 Glycoprotein protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-N-rFc

  Tag: N-rFc

  Domain: 21-298 aa of BC033830

  Sequence: QENQDGRYSLTYIYTGLSKHVEDVPAFQALGSLNDLQFFRYNSKDRKSQPMGLWRQVEGMEDWKQDSQLQKAREDIFMETLKDIVEYYNDSNGSHVLQGRFGCEIENNRSSGAFWKYYYDGKDYIEFNKEIPAWVPFDPAAQITKQKWEAEPVYVQRAKAYLEEECPATLRKYLKYSKNILDRQDPPSVVVTSHQAPGEKKKLKCLAYDFYPGKIDVHWTRAGEVQEPELRGDVLHNGNGTYQSWVVVAVPPQDTAPYSCHVQHSSLAQPLVVPWEAS

• Full Name: alpha-2-glycoprotein 1, zinc-binding
• Calculated Molecular Weight: 298 aa, 34 kDa
• Observed Molecular Weight: 41 kDa
• GenBank Accession Number: BC033830
• Gene Symbol: Zinc Alpha 2 Glycoprotein
• Gene ID (NCBI): 563
• RRID: AB_3745114
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P25311
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Zinc-alpha-2-glycoprotein (AZGP1) is a 41-kDa soluble protein normally found in body fluids, functions as a lipid mobilizing factor (PMID: 19188554). It is known to be expressed in the secretory epithelia of the liver, lung, breast, GI tract and sweat glands, sharing significant structural similarity with the class I major histocompatibility complex (MHC) antigens (PMID: 3422450). AZGP1 is involved in carcinogenesis and differentiation. Altered expression of AZGP1 has been reported in breast cancer, prostate cancer and lung adenocarcinoma, hepatocellular carcinoma, pancreatic carcinoma and oral tumors (PMID: 22625427).