Filter: AllWBIHCAffinity

WB analysis using 86808-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 86808-1-RR (TRAIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 86808-1-RR
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86808-1-RR (TRAIP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 86808-1-RR
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86808-1-RR (TRAIP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Affinity and Kinetic Characterization of 86808-1-RR
Biolayer interferometry (BLl) kinetic assays of 86808-1-RR against Human TRAIP were performed. The affinity constant is below 1 pM.

TRAIP Recombinant monoclonal antibody

Name: TRAIP Recombinant monoclonal antibody
Brand: Proteintech
SKU: 86808-1-RR

TRAIP Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 86808-1-RR

Clone No.251370C8

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

TRIP, EC:2.3.2.27, RING finger protein 206, RNF206, TRAF interacting protein

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, A549 cells
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86808-1-RR targets TRAIP in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag0491 Product name: Recombinant human TRAIP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-204 aa of BC000310 Sequence: MPIRALCTICSDFFDHSRDVAAIHCGHTFHLQCLIQWFETAPSRTCPQCRIQVGKRTIINKLFFDLAQEEENVLDAEFLKNELDNVRAQLSQKDKEKRDSQVIIDTLRDTLEERNATVVSLQQALGKAEMLCSTLKKQMKYLEQQQDETKQAQEEARRLRSKMKTMEQIELLLQSQRPEVEEMIRDMGVGQSAVEQLAVYCVSL Predict reactive species
Full Name	TRAF interacting protein
Calculated Molecular Weight	53 kDa
Observed Molecular Weight	53 kDa
GenBank Accession Number	BC000310
Gene Symbol	TRAIP
Gene ID (NCBI)	10293
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9BWF2
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

E3 ubiquitin-protein ligase TRAIP (TRAIP) is also named RING finger protein 206 (RNF206) and TRAF-interacting protein (TRIP). TRAIP, an E3 ligase containing a RING domain, has emerged as a significant contributor to maintaining genome integrity and TRAIP inhibits proliferation and migration of BLCA cells (PMID: 38123820). TRAIP helps replisomes overcome DNA interstrand crosslinks and DNA-protein crosslinks, whereas in mitosis it triggers disassembly of all replisomes that remain on chromatin (PMID: 33317933, PMID: 30842657).