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TRAIP Recombinant monoclonal antibody, PBS Only (86808-1-PBS)

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WB analysis using 86808-1-RR (same clone as 86808-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 86808-1-RR (TRAIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86808-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 86808-1-RR (same clone as 86808-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86808-1-RR (TRAIP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86808-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 86808-1-RR (same clone as 86808-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86808-1-RR (TRAIP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86808-1-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 86808-1-RR
Biolayer interferometry (BLl) kinetic assays of 86808-1-RR against Human TRAIP were performed. The affinity constant is below 1 pM.

TRAIP Recombinant monoclonal antibody, PBS Only

Name: TRAIP Recombinant monoclonal antibody, PBS Only
Brand: Proteintech
SKU: 86808-1-PBS

TRAIP Uni-rAb^® Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 86808-1-PBS

Clone No.251370C8

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

TRIP, EC:2.3.2.27, RING finger protein 206, RNF206, TRAF interacting protein

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

86808-1-PBS targets TRAIP in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag0491 Product name: Recombinant human TRAIP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-204 aa of BC000310 Sequence: MPIRALCTICSDFFDHSRDVAAIHCGHTFHLQCLIQWFETAPSRTCPQCRIQVGKRTIINKLFFDLAQEEENVLDAEFLKNELDNVRAQLSQKDKEKRDSQVIIDTLRDTLEERNATVVSLQQALGKAEMLCSTLKKQMKYLEQQQDETKQAQEEARRLRSKMKTMEQIELLLQSQRPEVEEMIRDMGVGQSAVEQLAVYCVSL Predict reactive species
Full Name	TRAF interacting protein
Calculated Molecular Weight	53 kDa
Observed Molecular Weight	53 kDa
GenBank Accession Number	BC000310
Gene Symbol	TRAIP
Gene ID (NCBI)	10293
RRID	AB_3745132
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9BWF2
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

E3 ubiquitin-protein ligase TRAIP (TRAIP) is also named RING finger protein 206 (RNF206) and TRAF-interacting protein (TRIP). TRAIP, an E3 ligase containing a RING domain, has emerged as a significant contributor to maintaining genome integrity and TRAIP inhibits proliferation and migration of BLCA cells (PMID: 38123820). TRAIP helps replisomes overcome DNA interstrand crosslinks and DNA-protein crosslinks, whereas in mitosis it triggers disassembly of all replisomes that remain on chromatin (PMID: 33317933, PMID: 30842657).