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  • Western Blot (WB) analysis of various lysates using COX6C Recombinant monoclonal antibody (86818-2-RR)

    WB analysis using 86818-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86818-2-RR (COX6C antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using COX6C Recombinant monoclonal antibody (86818-2-RR)

    WB analysis using 86818-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86818-2-RR (COX6C antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 86818-2-RR

    Affinity and Kinetic Characterization of 86818-2-RR

    Biolayer interferometry (BLl) kinetic assays of 86818-2-RR against Human COX6C were performed. The affinity constant is 1.43 nM.

COX6C Recombinant monoclonal antibody

COX6C Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 86818-2-RR
Clone No.251865D9

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

COX6C Recombinant monoclonal antibody, COX6C Recombinant monoclonal antibody, PBS Only (Capture)

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

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Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inHepG2 cells, mouse heart tissue, A-673 cells, MCF-7 cells, SW480 cells, rat heart tissue, human heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86818-2-RR targets COX6C in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag1982

Product name: Recombinant human COX6C protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-75 aa of BC000187

Sequence: MAPEVLPKPRMRGLLARRLRNHMAVAFVLSLGVAALYKFRVADQRKKAYADFYRNYDVMKDFEEMRKAGIFQSVK

Predict reactive species
Full Name cytochrome c oxidase subunit VIc
Calculated Molecular Weight 75 aa, 9 kDa
Observed Molecular Weight9 kDa
GenBank Accession NumberBC000187
Gene Symbol COX6C
Gene ID (NCBI) 1345
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP09669
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cytochrome c oxidase (COX) is the terminal oxidase in the respiratory chain in human and other mammalian cells. The enzyme is composed of 13 different subunits. COX6C(Cytochrome c oxidase subunit 6C) is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.

Protocols

Product Specific Protocols
WB protocol for COX6C antibody 86818-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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