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CAMK2B Recombinant monoclonal antibody, PBS Only (Detector) (86858-1-PBS)

Filter: AllWBSandwich ELISA sample testSandwich ELISAAffinity

WB analysis of U-251 using 86858-1-RR (same clone as 86858-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 86858-1-RR (CAMK2B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86858-1-PBS in a different storage buffer formulation.
Sandwich ELISA sample test of MP02919-1
The mean CAMK2B concentration was determined to be 130.09 ng/mL in SH-SY5Y cell extract based on a 1.20 mg/mL extract load, 669.36 ng/mL in HEK-293T cell extract based on a 1.20 mg/mL extract load, 436.22 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load, 332.36 ng/mL in A431 cell extract based on a 1.20 mg/mL extract load and 124.24 ng/mL in A549 cell extract based on a 1.20 mg/mL extract load.
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The mean CAMK2B concentration was determined to be 130.09 ng/mL in SH-SY5Y cell extract based on a 1.20 mg/mL extract load, 669.36 ng/mL in HEK-293T cell extract based on a 1.20 mg/mL extract load, 436.22 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load, 332.36 ng/mL in A431 cell extract based on a 1.20 mg/mL extract load and 124.24 ng/mL in A549 cell extract based on a 1.20 mg/mL extract load.
Sandwich ELISA standard curve of MP02919-1
Sandwich ELISA standard curve of MP02919-1, Human CAMK2B Recombinant Matched Antibody Pair - PBS only. 86858-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2119. 86858-1-PBS was HRP conjugated as the detection antibody. Range: 0.625-20 ng/mL.
Affinity and Kinetic Characterization of 86858-1-RR
Biolayer interferometry (BLl) kinetic assays of 86858-1-RR against Human CAMK2B were performed. The affinity constant is below 1 pM.

CAMK2B Recombinant monoclonal antibody, PBS Only (Detector)

Name: CAMK2B Recombinant monoclonal antibody, PBS Only (Detector)
Brand: Proteintech
SKU: 86858-1-PBS

CAMK2B Uni-rAb^® Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA

Cat No. 86858-1-PBS

Clone No.251925D4

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Sandwich ELISA, Indirect ELISA

Synonyms

Calcium/calmodulin-dependent protein kinase type II subunit beta, CaM kinase II subunit beta, CAM2, CaMK II subunit beta, CAMK2

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

86858-1-PBS targets CAMK2B as part of a matched antibody pair:

MP02919-1: 86858-2-PBS capture and 86858-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag2119 Product name: Recombinant human CAMK2B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-315 aa of BC019070 Sequence: MATTVTCTRFTDEYQLYEDIGKGAFSVVRRCVKLCTGHEYAAKIINTKKLSARDHQKLEREARICRLLKHSNIVRLHDSISEEGFHYLVFDLVTGGELFEDIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKCKGAAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLRKEAYGKPVDIWACGVILYILLVGYPPFWDEDQHKLYQQIKAGAYDFPSPEWDTVTPEAKNLINQMLTINPAKRITAHEALKHPWVCQRSTVASMMHRQETVECLKKFNARRKLKGAILTTMLATRNFS Predict reactive species
Full Name	calcium/calmodulin-dependent protein kinase II beta
Calculated Molecular Weight	503 aa, 56 kDa
Observed Molecular Weight	50-70 kDa
GenBank Accession Number	BC019070
Gene Symbol	CaMKII beta
Gene ID (NCBI)	816
RRID	AB_3745169
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q13554
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

CAMK2B(Calcium/calmodulin-dependent protein kinase type II subunit beta ), also named CAM2, CAMK2, and CAMKB, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, and CaMK subfamily. It is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. CAMK2B is a member of the NMDAR signaling complex in excitatory synapses, it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. It plays a distinct role in the induction of anergy in T lymphocytes, by differential regulation of IL10 and IL2 gene transcription suggesting MEF2A as a molecular target that can integrate different calcium signals(PMID:22578382). This protein has 8 isoforms produced by alternative splicing with a molecular weight between 50 kDa and 73 kDa. This antibody may have cross-reaction with CAMK2A/D/G due to the high homology.