CAMK2B Recombinant monoclonal antibody

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  WB analysis of U-251 using 86858-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86858-1-RR (CAMK2B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86858-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86858-1-RR against Human CAMK2B were performed. The affinity constant is below 1 pM.

CAMK2B Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86858-1-RR

Clone No.251925D4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Calcium/calmodulin-dependent protein kinase type II subunit beta, CaM kinase II subunit beta, CAM2, CaMK II subunit beta, CAMK2

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Tested Applications

• Positive WB detected in: U-251 cells, SH-SY5Y cells, Neuro-2a cells, C6 cells, rat brain tissue, mouse brain tissue, fetal human brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86858-1-RR targets CAMK2B in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag2119

  Product name: Recombinant human CAMK2B protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-315 aa of BC019070

  Sequence: MATTVTCTRFTDEYQLYEDIGKGAFSVVRRCVKLCTGHEYAAKIINTKKLSARDHQKLEREARICRLLKHSNIVRLHDSISEEGFHYLVFDLVTGGELFEDIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKCKGAAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLRKEAYGKPVDIWACGVILYILLVGYPPFWDEDQHKLYQQIKAGAYDFPSPEWDTVTPEAKNLINQMLTINPAKRITAHEALKHPWVCQRSTVASMMHRQETVECLKKFNARRKLKGAILTTMLATRNFS

• Full Name: calcium/calmodulin-dependent protein kinase II beta
• Calculated Molecular Weight: 503 aa, 56 kDa
• Observed Molecular Weight: 50-70 kDa
• GenBank Accession Number: BC019070
• Gene Symbol: CaMKII beta
• Gene ID (NCBI): 816
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q13554
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CAMK2B(Calcium/calmodulin-dependent protein kinase type II subunit beta ), also named CAM2, CAMK2, and CAMKB, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, and CaMK subfamily. It is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. CAMK2B is a member of the NMDAR signaling complex in excitatory synapses, it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. It plays a distinct role in the induction of anergy in T lymphocytes, by differential regulation of IL10 and IL2 gene transcription suggesting MEF2A as a molecular target that can integrate different calcium signals(PMID:22578382). This protein has 8 isoforms produced by alternative splicing with a molecular weight between 50 kDa and 73 kDa. This antibody may have cross-reaction with CAMK2A/D/G due to the high homology.