Tested Applications
| Positive WB detected in | mouse small intestine tissue, SH-SY5Y cells, rat small intestine tissue, mouse liver tissue, rat liver tissue, mouse kidney tissue, rat kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86878-1-RR targets TPK1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag1404 Product name: Recombinant human TPK1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 11-243 aa of BC014552 Sequence: LLSTGNLKYCLVILNQPLDNYFRHLWNKALLRACADGGANRLYDITEGERESFLPEFINGDFDSIRPEVREYYATKGCELISTPDQDHTDFTKCLKMLQKKIEEKDLKVDVIVTLGGLAGRFDQIMASVNTLFQATHITPFPIIIIQEESLIYLLQPGKHRLHVDTGMEGDWCGLIPVGQPCMQVTTTGLKWNLTNDVLAFGTLVSTSNTYDGSGVVTVETDHPLLWTMAIKS Predict reactive species |
| Full Name | thiamin pyrophosphokinase 1 |
| Calculated Molecular Weight | 28 kDa |
| Observed Molecular Weight | 28 kDa |
| GenBank Accession Number | BC014552 |
| Gene Symbol | TPK1 |
| Gene ID (NCBI) | 27010 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9H3S4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TPK1(Thiamin pyrophosphokinase 1) is also named as PP20(placental protein 20) and belongs to the thiamine pyrophosphokinase family. It is a cellular enzyme involved in the regulation of thiamine metabolism and catalyzes the conversion of thiamine, a form of vitamin B1, to thiamine pyrophosphate (TDP, or TPP). There is no difference in TPK1 expression in cultured fibroblasts from normal subjects or from patients with thiamine-responsive megaloblastic anemia(PMID:11342111). It can exsit as a dimer(PMID:21552434). Defects in TPK1 are the cause of thiamine metabolism dysfunction syndrome type 5, episodic encephalopathy type (THMD5).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TPK1 antibody 86878-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
