TPK1 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 86878-1-RR (same clone as 86878-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86878-1-RR (TPK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86878-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 86878-1-RR (same clone as 86878-1-PBS)

  SH-SY5Y cell lysates were subjected to SDS PAGE followed by western blot with 86878-1-RR (TPK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86878-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 86878-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86878-1-RR against Human TPK1 were performed. The affinity constant is below 1 pM.

TPK1 Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 86878-1-PBS

Clone No.251914G4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

EC:2.7.6.2, hTPK1, Placental protein 20, PP20, Thiamin pyrophosphokinase 1

---

Product Information

86878-1-PBS targets TPK1 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag1404

  Product name: Recombinant human TPK1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 11-243 aa of BC014552

  Sequence: LLSTGNLKYCLVILNQPLDNYFRHLWNKALLRACADGGANRLYDITEGERESFLPEFINGDFDSIRPEVREYYATKGCELISTPDQDHTDFTKCLKMLQKKIEEKDLKVDVIVTLGGLAGRFDQIMASVNTLFQATHITPFPIIIIQEESLIYLLQPGKHRLHVDTGMEGDWCGLIPVGQPCMQVTTTGLKWNLTNDVLAFGTLVSTSNTYDGSGVVTVETDHPLLWTMAIKS

• Full Name: thiamin pyrophosphokinase 1
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC014552
• Gene Symbol: TPK1
• Gene ID (NCBI): 27010
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9H3S4
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TPK1(Thiamin pyrophosphokinase 1) is also named as PP20(placental protein 20) and belongs to the thiamine pyrophosphokinase family. It is a cellular enzyme involved in the regulation of thiamine metabolism and catalyzes the conversion of thiamine, a form of vitamin B1, to thiamine pyrophosphate (TDP, or TPP). There is no difference in TPK1 expression in cultured fibroblasts from normal subjects or from patients with thiamine-responsive megaloblastic anemia(PMID:11342111). It can exsit as a dimer(PMID:21552434). Defects in TPK1 are the cause of thiamine metabolism dysfunction syndrome type 5, episodic encephalopathy type (THMD5).