Tested Applications
| Positive WB detected in | HepG2 cells |
| Positive IHC detected in | human placenta tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86913-6-RR targets Fibrinogen Alpha Chain in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag33674 Product name: Recombinant human FGA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 20-218 aa of BC020764 Sequence: ADSGEGDFLAEGGGVRGPRVVERHQSACKDSDWPFCSGEDWNYKCPSGCRMKGLIDEVNQDFTNRINKLKNSLFEYQKNNKDSHSLTTNIMEILRGDFSSANNRDNTYNRVSEDLRSRIEVLKRKVIEKVTANNLLVARVTTEETPHLKARAIKWQMRPEVKPIMKEHIAPREAMLNLALSEVSTLLLWGSLPCPPRLS* Predict reactive species |
| Full Name | fibrinogen alpha chain |
| Calculated Molecular Weight | 866 aa, 95 kDa |
| Observed Molecular Weight | 95kDa, 70 kDa |
| GenBank Accession Number | BC020764 |
| Gene Symbol | Fibrinogen Alpha Chain |
| Gene ID (NCBI) | 2243 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P02671 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Fibrinogen is a soluble plasma glycoprotein synthesized in the liver. It is composed of two sets of three structurally different subunits: alpha (FGA), beta (FGB), gamma (FGG). Fibrinogen is converted by thrombin into fibrin during blood coagulation. Fibrinogen and fibrin play overlapping roles in blood clotting, fibrinolysis, cellular and matrix interactions, the inflammatory response, wound healing, and neoplasia (PMID: 16102057). FGA is the alpha chain of fibrinogen. During conversion of fibrinogen to fibrin, fibrinopeptide A is cleaved from FGA by thrombin.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Fibrinogen Alpha Chain antibody 86913-6-RR | Download protocol |
| WB protocol for Fibrinogen Alpha Chain antibody 86913-6-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
