Fibrinogen Alpha Chain Recombinant monoclonal antibody, PBS Only

• Home
• Products
• Primary Antibodies
• Recombinant Antibodies
• Fibrinogen Alpha Chain Recombinant monoclonal antibody, PBS Only (86913-6-PBS)

Fibrinogen Alpha Chain Uni-rAb^® Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 86913-6-PBS

Clone No.260891B12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

FGA, Fib2, Fibrinopeptide A

• 

  WB analysis using 86913-6-RR (same clone as 86913-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86913-6-RR (FGA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86913-6-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 86913-6-RR (same clone as 86913-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 86913-6-RR (Fibrinogen Alpha Chain antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86913-6-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 86913-6-RR (same clone as 86913-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 86913-6-RR (Fibrinogen Alpha Chain antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86913-6-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

---

Product Information

86913-6-PBS targets Fibrinogen Alpha Chain in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag33674

  Product name: Recombinant human FGA protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 20-218 aa of BC020764

  Sequence: ADSGEGDFLAEGGGVRGPRVVERHQSACKDSDWPFCSGEDWNYKCPSGCRMKGLIDEVNQDFTNRINKLKNSLFEYQKNNKDSHSLTTNIMEILRGDFSSANNRDNTYNRVSEDLRSRIEVLKRKVIEKVTANNLLVARVTTEETPHLKARAIKWQMRPEVKPIMKEHIAPREAMLNLALSEVSTLLLWGSLPCPPRLS*

• Full Name: fibrinogen alpha chain
• Calculated Molecular Weight: 866 aa, 95 kDa
• Observed Molecular Weight: 95kDa, 70 kDa
• GenBank Accession Number: BC020764
• Gene Symbol: Fibrinogen Alpha Chain
• Gene ID (NCBI): 2243
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P02671
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Fibrinogen is a soluble plasma glycoprotein synthesized in the liver. It is composed of two sets of three structurally different subunits: alpha (FGA), beta (FGB), gamma (FGG). Fibrinogen is converted by thrombin into fibrin during blood coagulation. Fibrinogen and fibrin play overlapping roles in blood clotting, fibrinolysis, cellular and matrix interactions, the inflammatory response, wound healing, and neoplasia (PMID: 16102057). FGA is the alpha chain of fibrinogen. During conversion of fibrinogen to fibrin, fibrinopeptide A is cleaved from FGA by thrombin.