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JAM3 Recombinant monoclonal antibody, PBS Only (Detector) (86960-1-PBS)

Filter: AllWBIF/ICCCytometric bead array sample testCytometric bead arrayAffinity

WB analysis using 86960-1-RR (same clone as 86960-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 86960-1-RR (JAM3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86960-1-PBS in a different storage buffer formulation.
IF Staining of U-251 using 86960-1-RR (same clone as 86960-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed U-251 cells using JAM3 antibody (86960-1-RR, Clone: 251954C5) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86960-1-PBS in a different storage buffer formulation.
Cytometric bead array sample test of MP02796-1
The mean JAM3 concentration was determined to be 23.0 ng/mL in Jurkat cell extract based on a 1.2 mg/mL extract load, 30.4 ng/mL in HEK-293 cell extract based on a 1.2 mg/mL extract load, HeLa cell extract were served as a negative control.
Cytometric bead array standard curve of MP02796-1
Cytometric bead array standard curve of MP02796-1, JAM3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86960-2-PBS. Detection antibody: 86960-1-PBS. Standard: Eg6611. Range: 0.156-20 ng/mL.
Affinity and Kinetic Characterization of 86960-1-RR
Biolayer interferometry (BLl) kinetic assays of 86960-1-RR against Human JAM3 were performed. The affinity constant is 0.527 nM.

JAM3 Recombinant monoclonal antibody, PBS Only (Detector)

Name: JAM3 Recombinant monoclonal antibody, PBS Only (Detector)
Brand: Proteintech
SKU: 86960-1-PBS

JAM3 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 86960-1-PBS

Clone No.251954C5

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

JAM-2, JAM-3, JAM-C, Junctional adhesion molecule 3, Junctional adhesion molecule C

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

86960-1-PBS targets JAM3 as part of a matched antibody pair:

MP02796-1: 86960-2-PBS capture and 86960-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg6611 Product name: Recombinant Human JAM3 protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 32-241 aa of NM_032801.5 Sequence: VNLKSSNRTPVVQEFESVELSCIITDSQTSDPRIEWKKIQDEQTTYVFFDNKIQGDLAGRAEILGKTSLKIWNVTRRDSALYRCEVVARNDRKEIDEIVIELTVQVKPVTPVCRVPKAVPVGKMATLHCQESEGHPRPHYSWYRNDVPLPTDSRANPRFRNSSFHLNSETGTLVFTAVHKDDSGQYYCIASNDAGSARCEEQEMEVYDLN Predict reactive species
Full Name	junctional adhesion molecule 3
Calculated Molecular Weight	35 kDa
Observed Molecular Weight	40 kDa
GenBank Accession Number	NM_032801.5
Gene Symbol	JAM3
Gene ID (NCBI)	83700
RRID	AB_3745236
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9BX67-1
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Junctional adhesion molecule 3 (JAM3) is a type I transmembrane glycoprotein containing two Ig-like domains, which is expressed in various tissues and plays a crucial role in cell junctions, cell polarity, and motility (PMID: 34292449; 28931565). It has been proposed that JAM3 participates in leukocyte-platelet interactions, as well as angiogenesis and brain development (PMID: 12208882; 23255084). JAM3 may also be a new marker for predicting the prognosis and immune functions of bladder cancer patients (PMID: 38400838).