Tested Applications
| Positive WB detected in | Staurosporine treated Jurkat cells |
| Positive IF/ICC detected in | Staurosporine treated Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87055-4-RR targets Cleaved Caspase 3/P12 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | caspase 3, apoptosis-related cysteine peptidase |
| Calculated Molecular Weight | 277 aa, 32 kDa |
| Observed Molecular Weight | 12 kDa |
| GenBank Accession Number | BC016926 |
| Gene Symbol | Caspase 3 |
| Gene ID (NCBI) | 836 |
| RRID | AB_3745305 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P42574 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Cleaved Caspase-3 p12 is one of the two subunits of active Caspase-3 and plays a key catalytic role in the execution of apoptosis. Precursor cleavage of Caspase-3 ultimately forms an active heterotetramer composed of two p17 subunits and two p12 subunits.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Cleaved Caspase 3/P12 antibody 87055-4-RR | Download protocol |
| WB protocol for Cleaved Caspase 3/P12 antibody 87055-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Cleaved Caspase 3/P12 Recombinant monoclonal antibody (252012D12), catalog number 87055-4-RR, has 5 total citations. These appear in Advanced Science, Chinese Journal of Natural Medicines, Redox Report, Biochemical Genetics, and Drug Design, Development and Therapy. The research fields span intervertebral disc degeneration, triple-negative breast cancer, ferroptosis and sepsis-associated acute kidney injury, LPS-induced acute kidney injury, and corneal epithelial damage in dry eye disease.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Elevated TRIM25 Impairs Poly (ADP-ribose) Metabolism via PARG Degradation and Mediates Compression-Induced Intervertebral Disc Degeneration. | ||
Chin J Nat Med Ramulus Mori alkaloids (SZ-A) sensitize triple-negative breast cancer to cisplatin via PLA2G2A-mediated ceramide metabolism. | ||
Redox Rep Sirtuin 5-mediated desuccinylation of PRDX6 inhibits ferroptosis and alleviates sepsis-associated acute kidney injury. | ||
Biochem Genet MiR-146b-5p/ERBB4 Axis Drives LPS-induced Acute Kidney Injury by Modulating NF-κB/p65. | ||
Drug Des Devel Ther Honokiol Alleviates Dry Eye-Related Corneal Epithelial Damage by Activating SIRT3/PINK1-Mediated Mitophagy. |
