Filter: AllWBSandwich ELISA sample testSandwich ELISAIF/ICCCytometric bead array sample testCytometric bead arrayAffinity

WB analysis using 87056-2-RR (same clone as 87056-2-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 87056-2-RR (Integrin alpha-1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87056-2-PBS in a different storage buffer formulation.
WB analysis of human placenta using 87056-2-RR (same clone as 87056-2-PBS)
human placenta tissue were subjected to SDS PAGE followed by western blot with 87056-2-RR (Integrin alpha-1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87056-2-PBS in a different storage buffer formulation.
Sandwich ELISA sample test of MP02854-2
The mean Integrin alpha 1 concentration was determined to be 241.89 ng/mL in HepG2 cell extract based on a 1.20 mg/mL extract load, 523.16 ng/mL in HuH-7 cell extract based on a 1.20 mg/mL extract load, 15.42 ng/mL in K-562 cell extract based on a 1.20 mg/mL extract load and 17.42 ng/mL in U-937 cell extract based on a 1.20 mg/mL extract load.
Sandwich ELISA sample test of MP02854-2
Plasma of eight individual healthy human donors was measured. The Integrin alpha 1 concentration of detected samples was determined to be 84.94 ng/mL with a range of 50.93-128.02 ng/mL.
Sandwich ELISA standard curve of MP02854-2
Sandwich ELISA standard curve of MP02854-2, Human Integrin alpha 1 Recombinant Matched Antibody Pair - PBS only. 87056-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2089. 87056-4-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.
IF Staining of HeLa using 87056-2-RR (same clone as 87056-2-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Integrin alpha 1 antibody (87056-2-RR, Clone: 251984B7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87056-2-PBS in a different storage buffer formulation.
Cytometric bead array sample test of MP02854-1
The mean Integrin alpha-1 concentration was determined to be 51.4 ng/mL in human plasma.
Cytometric bead array standard curve of MP02854-1
Cytometric bead array standard curve of MP02854-1, Integrin alpha-1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 87056-2-PBS. Detection antibody: 87056-1-PBS. Standard: Eg2089. Range: 0.781-100 ng/mL.
Affinity of 87056-2-PBS
Biolayer interferometry (BLl) kinetic assay of 87056-2-PBS against Human Integrin alpha-1 was performed. The affinity constant is 1.16 nM.
Affinity of 87056-2-RR
Biolayer interferometry (BLl) kinetic assays of 87056-2-RR against Human Integrin alpha 1 were performed. The affinity constant is 0.468 nM.

Integrin alpha 1 Recombinant monoclonal antibody, PBS Only (Capture)

Name: Integrin alpha 1 Recombinant monoclonal antibody, PBS Only (Capture)
Brand: Proteintech
SKU: 87056-2-PBS

Integrin alpha 1 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Cat No. 87056-2-PBS

Clone No.251984B7

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

ITGA1, CD49 antigen-like family member A, CD49a, integrin, alpha 1, Laminin and collagen receptor

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

87056-2-PBS targets Integrin alpha 1 as part of a matched antibody pair:

MP02854-1: 87056-2-PBS capture and 87056-1-PBS detection (validated in Cytometric bead array)

MP02854-2: 87056-2-PBS capture and 87056-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg2089 Product name: Recombinant Human Integrin alpha-1 protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 29-1141 aa of BC137121 Sequence: FNVDVKNSMTFSGPVEDMFGYTVQQYENEEGKWVLIGSPLVGQPKNRTGDVYKCPVGRGESLPCVKLDLPVNTSIPNVTEVKENMTFGSTLVTNPNGGFLACGPLYAYRCGHLHYTTGICSDVSPTFQVVNSIAPVQECSTQLDIVIVLDGSNSIYPWDSVTAFLNDLLERMDIGPKQTQVGIVQYGENVTHEFNLNKYSSTEEVLVAAKKIVQRGGRQTMTALGIDTARKEAFTEARGARRGVKKVMVIVTDGESHDNHRLKKVIQDCEDENIQRFSIAILGSYNRGNLSTEKFVEEIKSIASEPTEKHFFNVSDELALVTIVKTLGERIFALEATADQSAASFEMEMSQTGFSAHYSQDWVMLGAVGAYDWNGTVVMQKASQIIIPRNTTFNVESTKKNEPLASYLGYTVNSATASSGDVLYIAGQPRYNHTGQVIIYRMEDGNIKILQTLSGEQIGSYFGSILTTTDIDKDSNTDILLVGAPMYMGTEKEEQGKVYVYALNQTRFEYQMSLEPIKQTCCSSRQHNSCTTENKNEPCGARFGTAIAAVKDLNLDGFNDIVIGAPLEDDHGGAVYIYHGSGKTIRKEYAQRIPSGGDGKTLKFFGQSIHGEMDLNGDGLTDVTIGGLGGAALFWSRDVAVVKVTMNFEPNKVNIQKKNCHMEGKETVCINATVCFDVKLKSKEDTIYEADLQYRVTLDSLRQISRSFFSGTQERKVQRNITVRKSECTKHSFYMLDKHDFQDSVRITLDFNLTDPENGPVLDDSLPNSVHEYIPFAKDCGNKEKCISDLSLHVATTEKDLLIVRSQNDKFNVSLTVKNTKDSAYNTRTIVHYSPNLVFSGIEAIQKDSCESNHNITCKVGYPFLRRGEMVTFKILFQFNTSYLMENVTIYLSATSDSEEPPETLSDNVVNISIPVKYEVGLQFYSSASEYHISIAANETVPEVINSTEDIGNEINIFYLIRKSGSFPMPELKLSISFPNMTSNGYPVLYPTGLSSSENANCRPHIFEDPFSINSGKKMTTSTDHLKRGTILDCNTCKFATITCNLTSSDISQVNVSLILWKPTFIKSYFSSLNLTIRGELRSENASLVLSSSNQKRELAIQISKDGLPGRVP Predict reactive species
Full Name	integrin, alpha 1
Calculated Molecular Weight	1179 aa, 131 kDa
Observed Molecular Weight	200 kDa
GenBank Accession Number	BC137121
Gene Symbol	Integrin alpha 1
Gene ID (NCBI)	3672
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P56199
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID:19118207). Integrin alpha-1 (ITGA1, CD49a) combines with the beta 1 subunit (ITGB1, CD29) to form a cell-surface receptor for collagen and laminin. This receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. Integrin alpha-1 is a transmembrane glycoprotein with an apparent molecular weight of 180-250 kDa, larger than the calculated molecular weight of 131 kDa (PMID: 2475259; 3257425; 11557277; 18840653).