PTH1R Recombinant monoclonal antibody

PTH1R Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87076-3-RR

Clone No. 252173F3

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Parathyroid hormone/parathyroid hormone-related peptide receptor, PFE, PTHR, PTHR1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

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WB analysis using 87076-3-RR
Various lysates were subjected to SDS PAGE followed by western blot with 87076-3-RR (PTH1R antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HEK-293 using 87076-3-RR
WB result of PTH1R antibody (87076-3-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTH1R transfected HEK-293 cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse kidney using 87076-3-RR
Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 87076-3-RR (PTH1R antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
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Tested Applications

Positive WB detected in

HeLa cells, HEK-293 cells, mouse kidney tissue, HepG2 cells, MCF-7 cells, U2OS cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87076-3-RR targets PTH1R in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg6501 Product name: Recombinant Human PTH1R protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 27-188 aa of NM_000316.2 Sequence: DADDVMTKEEQIFLLHRAQAQCEKRLKEVLQRPASIMESDKGWTSASTSGKPRKDKASGKLYPESEEDKEAPTGSRYRGRPCLPEWDHILCWPLGAPGEVVAVPCPDYIYDFNHKGHAYRRCDRNGSWELVPGHNRTWANYSECVKFLTNETREREVFDRLG Predict reactive species
Full Name	parathyroid hormone 1 receptor
Calculated Molecular Weight	66 kDa
Observed Molecular Weight	66 kDa and 90 kDa
GenBank Accession Number	NM_000316.2
Gene Symbol	PTH1R
Gene ID (NCBI)	5745
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q03431
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTH1R is a receptor for parathyroid hormone and parathyroid hormone-related peptide (PTHrP). PTH1R is a glycoprotein with seven transmembrane domains and it has an extracellular N-terminus region and an intracellular C-terminus region. The activity of PTH1R is mediated by G proteins which activate adenylyl cyclase. It has been reported that PTHrP and PTH1R are associated with the differentiation of bone and cartilage in the developing stages. The calculated molecular weight of PTH1R is 66 kDa, and PTH1R can form a 120 kDa dimer in the non-reducing condition. With glycosylation modification, the molecular weight of PTH1R will migrate to 90 kDa (PMID: 16783882).