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PPP6C Recombinant monoclonal antibody (87155-1-RR)

PPP6C Recombinant monoclonal antibody

PPP6C Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87155-1-RR

Clone No.252251E9

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

EC:3.1.3.16, PP6C, PPP6, Serine/threonine-protein phosphatase 6 catalytic subunit, Serine/threonine-protein phosphatase 6 catalytic subunit, N-terminally processed

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

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Freight/Packing: -

Quantity

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Filter: AllWBIF/ICCAffinity

WB analysis using 87155-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 87155-1-RR (PPP6C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HEK-293 using 87155-1-RR
Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using PPP6C antibody (87155-1-RR, Clone: 252251E9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Affinity of 87155-1-RR
Biolayer interferometry (BLl) kinetic assays of 87155-1-RR against Human PPP6C were performed. The affinity constant is 49.7 nM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in

HeLa cells, HepG2 cells, HEK-293T cells, Jurkat cells, NIH/3T3 cells, mouse testis tissue, rat testis tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87155-1-RR targets PPP6C in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag8554 Product name: Recombinant human PPP6C protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-305 aa of BC006990 Sequence: MAPLDLDKYVEIARLCKYLPENDLKRLCDYVCDLLLEESNVQPVSTPVTVCGDIHGQFYDLCELFRTGGQVPDTNYIFMGDFVDRGYYSLETFTYLLALKAKWPDRITLLRGNHESRQITQVYGFYDECQTKYGNANAWRYCTKVFDMLTVAALIDEQILCVHGGLSPDIKTLDQIRTIERNQEIPHKGAFCDLVWSDPEDVDTWAISPRGAGWLFGAKVTNEFVHINNLKLICRAHQLVHEGYKFMFDEKLVTVWSAPNYCYRCGNIASIMVFKDVNTREPKLFRAVPDSERVIPPRTTTPYFL Predict reactive species
Full Name	protein phosphatase 6, catalytic subunit
Calculated Molecular Weight	305 aa, 35 kDa
Observed Molecular Weight	30-35 kDa
GenBank Accession Number	BC006990
Gene Symbol	PPP6C
Gene ID (NCBI)	5537
RRID	AB_3745376
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	O00743
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPP6C (Protein Phosphatase 6 Catalytic Subunit or simply PP6) is a highly conserved serine/threonine phosphatase belonging to the PP2A-like subfamily. This enzyme complex plays critical roles in a wide array of fundamental cellular processes, including cell cycle progression, DNA damage response, NF-κB signaling, and microtubule organization. PPP6C mutations are found across multiple cancer types but are most common in melanoma and other skin cancers (PMID: 33789117).