FAM84B Recombinant monoclonal antibody

FAM84B Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 87176-3-RR

Clone No.252473G6

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

LRAT domain-containing 2, LRATD2, NSE2, Protein FAM84B, Protein LRATD2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

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Freight/Packing: -

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Filter: AllWBIF/ICCAffinity

WB analysis of MCF-7 using 87176-3-RR
WB result of FAM84B antibody (87176-3-RR; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FAM84B transfected MCF-7 cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 87176-3-RR
Various lysates were subjected to SDS PAGE followed by western blot with 87176-3-RR (FAM84B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 87176-3-RR
Various lysates were subjected to SDS PAGE followed by western blot with 87176-3-RR (FAM84B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of MCF-7 using 87176-3-RR
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using FAM84B antibody (87176-3-RR, Clone: 252473G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Affinity of 87176-3-RR
Biolayer interferometry (BLl) kinetic assays of 87176-3-RR against Human FAM84B were performed. The affinity constant is 4.50 nM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	MCF-7 cells, mouse stomach tissue, MDA-MB-453 cells, rat stomach tissue
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87176-3-RR targets FAM84B in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag13243 Product name: Recombinant human FAM84B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-310 aa of BC052957 Sequence: MGNQVEKLTHLSYKEVPTADPTGVDRDDGPRIGVSYIFSNDDEDVEPQPPPQGPDGGGLPDGGDGPPPPQPQPYDPRLHEVECSVFYRDECIYQKSFAPGSAALSTYTPENLLNKCKPGDLVEFVSQAQYPHWAVYVGNFQVVHLHRLEVINSFLTDASQGRRGRVVNDLYRYKPLSSSAVVRNALAHVGAKERELSWRNSESFAAWCRYGKREFKIGGELRIGKQPYRLQIQLSAQRSHTLEFQSLEDLIMEKRRNDQIGRAAVLQELATHLHPAEPEEGDSNVARTTPPPGRPPAPSSEEEDGEAVAH Predict reactive species
Full Name	family with sequence similarity 84, member B
Calculated Molecular Weight	34 kDa
Observed Molecular Weight	40 kDa
GenBank Accession Number	BC052957
Gene Symbol	FAM84B
Gene ID (NCBI)	157638
RRID	AB_3745391
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q96KN1
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FAM84B (Family with sequence similarity 84 member B) is a protein-coding gene located on human chromosome 8. Although its full physiological function remains under investigation, accumulating evidence links FAM84B to cell proliferation, cytoskeletal organization and cancer progression. In several tumor types-most notably prostate, esophageal squamous-cell, breast and glioma-FAM84B is frequently amplified or over-expressed, and high expression often correlates with advanced stage, higher Gleason score and poorer patient survival.