FAM84B Recombinant monoclonal antibody, PBS Only

• Home
• Products
• Primary Antibodies
• Recombinant Antibodies
• FAM84B Recombinant monoclonal antibody, PBS Only (87176-3-PBS)

FAM84B Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 87176-3-PBS

Clone No.252473G6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

LRAT domain-containing 2, LRATD2, NSE2, Protein FAM84B, Protein LRATD2

• 

  WB analysis of MCF-7 using 87176-3-RR (same clone as 87176-3-PBS)

  WB result of FAM84B antibody (87176-3-RR; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FAM84B transfected MCF-7 cells. This data was developed using the same antibody clone with 87176-3-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 87176-3-RR (same clone as 87176-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87176-3-RR (FAM84B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87176-3-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 87176-3-RR (same clone as 87176-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87176-3-RR (FAM84B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87176-3-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of MCF-7 using 87176-3-RR (same clone as 87176-3-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using FAM84B antibody (87176-3-RR, Clone: 252473G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87176-3-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity of 87176-3-RR

  Biolayer interferometry (BLl) kinetic assays of 87176-3-RR against Human FAM84B were performed. The affinity constant is 4.50 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

---

Product Information

87176-3-PBS targets FAM84B in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag13243

  Product name: Recombinant human FAM84B protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-310 aa of BC052957

  Sequence: MGNQVEKLTHLSYKEVPTADPTGVDRDDGPRIGVSYIFSNDDEDVEPQPPPQGPDGGGLPDGGDGPPPPQPQPYDPRLHEVECSVFYRDECIYQKSFAPGSAALSTYTPENLLNKCKPGDLVEFVSQAQYPHWAVYVGNFQVVHLHRLEVINSFLTDASQGRRGRVVNDLYRYKPLSSSAVVRNALAHVGAKERELSWRNSESFAAWCRYGKREFKIGGELRIGKQPYRLQIQLSAQRSHTLEFQSLEDLIMEKRRNDQIGRAAVLQELATHLHPAEPEEGDSNVARTTPPPGRPPAPSSEEEDGEAVAH

• Full Name: family with sequence similarity 84, member B
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC052957
• Gene Symbol: FAM84B
• Gene ID (NCBI): 157638
• RRID: AB_3745391
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96KN1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

FAM84B (Family with sequence similarity 84 member B) is a protein-coding gene located on human chromosome 8. Although its full physiological function remains under investigation, accumulating evidence links FAM84B to cell proliferation, cytoskeletal organization and cancer progression. In several tumor types-most notably prostate, esophageal squamous-cell, breast and glioma-FAM84B is frequently amplified or over-expressed, and high expression often correlates with advanced stage, higher Gleason score and poorer patient survival.