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Phospho-PKC Delta (Ser645) Recombinant monoclonal antibody (87213-1-RR)

Phospho-PKC Delta (Ser645) Recombinant monoclonal antibody

Phospho-PKC Delta (Ser645) Uni-rAb^® Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 87213-1-RR

Clone No.252421F11

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

Phospho-PKC Delta, Phospho-PKC Delta (Ser643), PKC Delta (Ser643), PKC Delta (Ser645), p-PKC Delta

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

- +

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Filter: AllWBFC (Intra)

WB analysis using 87213-1-RR
Non-treated HeLa cells, phosphatase inhibitor treated HeLa cells and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 87213-1-RR (Phospho-PKC Delta (Ser645) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 87213-1-RR
Non-treated A549 cells, phosphatase inhibitor treated A549 cells and λ phosphatase treated A549 cells were subjected to SDS PAGE followed by western blot with 87213-1-RR (Phospho-PKC Delta (Ser645) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of A549 using 87213-1-RR
1X10^6 A549 cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.13 ug Phospho-PKC Delta (Ser645) Recombinant monoclonal antibody (87213-1-RR, Clone:252421F11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1X10^6 A549 cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.13 ug Phospho-PKC Delta (Ser645) Recombinant monoclonal antibody (87213-1-RR, Clone:252421F11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	A549 cells, HeLa cells
Positive FC (Intra) detected in	λ phosphatase treated A549 cells

This antibody is equivalent to Ser643 in mice.

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87213-1-RR targets Phospho-PKC Delta (Ser645) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	protein kinase C, delta
Calculated Molecular Weight	78 kDa
Observed Molecular Weight	78 kDa
GenBank Accession Number	NM_006254
Gene Symbol	PKC Delta
Gene ID (NCBI)	5580
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q05655
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein kinase C (PKC) was initially identified and characterized as a protein hydrolysis-activated kinase called protein kinase M. It has been established that PKC is a family of at least 12 serine/threonine kinases that is divided into three subfamilies: The classical PKCs (α, β1, β2, and γ), which are activated by diacylglycerol (DAG) and calcium; the novel PKCs (δ, ε, η, and θ) which are activated by DAG; and the atypical PKCs (ζ and λ/ι), which respond to neither DAG nor calcium. Phospho-PKC Delta (Ser645) refers to the phosphorylated form of Protein Kinase C Delta (PKCδ) at serine residue 645. This phosphorylation site is located in the C-terminal tail of PKCδ, specifically in the turn motif region, and serves as a critical "priming" phosphorylation that regulates enzyme stability and catalytic activity. (PMID: 28784722) This antibody is equivalent to Ser643 in mice.

Protocols

Product Specific Protocols
FC protocol for Phospho-PKC Delta (Ser645) antibody 87213-1-RR	Download protocol
WB protocol for Phospho-PKC Delta (Ser645) antibody 87213-1-RR	Download protocol