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Phospho-PKC Delta (Ser645) Recombinant monoclonal antibody, PBS Only (87213-1-PBS)

Phospho-PKC Delta (Ser645) Recombinant monoclonal antibody, PBS Only

Name: Phospho-PKC Delta (Ser645) Recombinant monoclonal antibody, PBS Only, 100ug
Brand: Proteintech
SKU: 87213-1-PBS-100UG

Phospho-PKC Delta (Ser645) Uni-rAb^® Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Cat No. 87213-1-PBS

Clone No.252421F11

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

Phospho-PKC Delta, Phospho-PKC Delta (Ser643), PKC Delta (Ser643), PKC Delta (Ser645), p-PKC Delta

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

SKU:

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Freight/Packing: -

Quantity

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Filter: AllWBFC (Intra)

WB analysis using 87213-1-RR (same clone as 87213-1-PBS)
Non-treated HeLa cells, phosphatase inhibitor treated HeLa cells and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 87213-1-RR (Phospho-PKC Delta (Ser645) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 87213-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
Non-treated HeLa cells, phosphatase inhibitor treated HeLa cells and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 87213-1-RR (Phospho-PKC Delta (Ser645) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 87213-1-PBS in a different storage buffer formulation.
WB analysis using 87213-1-RR (same clone as 87213-1-PBS)
Non-treated A549 cells, phosphatase inhibitor treated A549 cells and λ phosphatase treated A549 cells were subjected to SDS PAGE followed by western blot with 87213-1-RR (Phospho-PKC Delta (Ser645) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 87213-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
Non-treated A549 cells, phosphatase inhibitor treated A549 cells and λ phosphatase treated A549 cells were subjected to SDS PAGE followed by western blot with 87213-1-RR (Phospho-PKC Delta (Ser645) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 87213-1-PBS in a different storage buffer formulation.
FC experiment of A549 using 87213-1-RR (same clone as 87213-1-PBS)
1X10^6 A549 cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.13 ug Phospho-PKC Delta (Ser645) Recombinant monoclonal antibody (87213-1-RR, Clone:252421F11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026). This data was developed using the same antibody clone with 87213-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1X10^6 A549 cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.13 ug Phospho-PKC Delta (Ser645) Recombinant monoclonal antibody (87213-1-RR, Clone:252421F11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026). This data was developed using the same antibody clone with 87213-1-PBS in a different storage buffer formulation.

Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

87213-1-PBS targets Phospho-PKC Delta (Ser645) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	protein kinase C, delta
Calculated Molecular Weight	78 kDa
Observed Molecular Weight	78 kDa
GenBank Accession Number	NM_006254
Gene Symbol	PKC Delta
Gene ID (NCBI)	5580
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q05655
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Protein kinase C (PKC) was initially identified and characterized as a protein hydrolysis-activated kinase called protein kinase M. It has been established that PKC is a family of at least 12 serine/threonine kinases that is divided into three subfamilies: The classical PKCs (α, β1, β2, and γ), which are activated by diacylglycerol (DAG) and calcium; the novel PKCs (δ, ε, η, and θ) which are activated by DAG; and the atypical PKCs (ζ and λ/ι), which respond to neither DAG nor calcium. Phospho-PKC Delta (Ser645) refers to the phosphorylated form of Protein Kinase C Delta (PKCδ) at serine residue 645. This phosphorylation site is located in the C-terminal tail of PKCδ, specifically in the turn motif region, and serves as a critical "priming" phosphorylation that regulates enzyme stability and catalytic activity. (PMID: 28784722) This antibody is equivalent to Ser643 in mice.