RPA2 Recombinant monoclonal antibody

RPA2 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 87254-1-RR

Clone No.252472D6

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

REPA2, Replication factor A protein 2, replication protein A2, 32kDa, RF-A protein 2, RP A p32

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

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Filter: AllWBIF/ICCAffinity

WB analysis using 87254-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 87254-1-RR (RPA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of A431 using 87254-1-RR
Immunofluorescent analysis of (4% PFA) fixed A431 cells using RPA2 antibody (87254-1-RR, Clone: 252472D6 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Affinity of 87254-1-RR
Biolayer interferometry (BLl) kinetic assays of 87254-1-RR against Human RPA were performed. The affinity constant is 1.22 nM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	rat testis tissue, HeLa cells, HEK-293 cells, Jurkat cells, MOLT-4 cells, THP-1 cells, mouse testis tissue
Positive IF/ICC detected in	A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunofluorescence (IF)/ICC	IF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87254-1-RR targets RPA2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag0690 Product name: Recombinant human RPA2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 80-270 aa of BC001630 Sequence: IRHAEKAPTNIVYKIDDMTAAPMDVRQWVDTDDTSSENTVVPPETYVKVAGHLRSFQNKKSLVAFKIMPLEDMNEFTTHILEVINAHMVLSKANSQPSAGRAPISNPGMSEAGNFGGNSFMPANGLTVAQNQVLNLIKACPRPEGLNFQDLKNQLKHMSVSSIKQAVDFLSNEGHIYSTVDDDHFKSTDAE Predict reactive species
Full Name	replication protein A2, 32kDa
Calculated Molecular Weight	29 kDa
Observed Molecular Weight	32 kDa
GenBank Accession Number	BC001630
Gene Symbol	RPA2
Gene ID (NCBI)	6118
RRID	AB_3745434
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P15927
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPA2, a subunit of the heterotrimeric replication protein A (RPA) complex, is overexpressed in various cancers. RPA2 is a major target for upstream kinases, including CDKs and the three PIKKs (ATR, ataxia-telangiectasia mutated [ATM], and DNA-PK). RPA2 comprises multiple critical Ser/Tr residues that are phosphorylated in sequential order in response to genotoxic stress.