RPA2 Recombinant monoclonal antibody, PBS Only

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• RPA2 Recombinant monoclonal antibody, PBS Only (87254-1-PBS)

RPA2 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 87254-1-PBS

Clone No.252472D6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

REPA2, Replication factor A protein 2, replication protein A2, 32kDa, RF-A protein 2, RP A p32

• 

  WB analysis using 87254-1-RR (same clone as 87254-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87254-1-RR (RPA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87254-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A431 using 87254-1-RR (same clone as 87254-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using RPA2 antibody (87254-1-RR, Clone: 252472D6 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 87254-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity of 87254-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87254-1-RR against Human RPA were performed. The affinity constant is 1.22 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

87254-1-PBS targets RPA2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag0690

  Product name: Recombinant human RPA2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 80-270 aa of BC001630

  Sequence: IRHAEKAPTNIVYKIDDMTAAPMDVRQWVDTDDTSSENTVVPPETYVKVAGHLRSFQNKKSLVAFKIMPLEDMNEFTTHILEVINAHMVLSKANSQPSAGRAPISNPGMSEAGNFGGNSFMPANGLTVAQNQVLNLIKACPRPEGLNFQDLKNQLKHMSVSSIKQAVDFLSNEGHIYSTVDDDHFKSTDAE

• Full Name: replication protein A2, 32kDa
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC001630
• Gene Symbol: RPA2
• Gene ID (NCBI): 6118
• RRID: AB_3745434
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P15927
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RPA2, a subunit of the heterotrimeric replication protein A (RPA) complex, is overexpressed in various cancers. RPA2 is a major target for upstream kinases, including CDKs and the three PIKKs (ATR, ataxia-telangiectasia mutated [ATM], and DNA-PK). RPA2 comprises multiple critical Ser/Tr residues that are phosphorylated in sequential order in response to genotoxic stress.