Tested Applications
| Positive WB detected in | HeLa cells, NIH/3T3 cells, RAW 264.7 cells, PC-12 cells |
| Positive IF/ICC detected in | λ phosphatase treated HeLa cells |
| Positive FC (Intra) detected in | λ phosphatase treated HeLa cells |
This antibody recognizes the phosphorylation at the Thr198 site of PKA-C (including PRKACA, PRKACB, and PRKACG).
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87365-3-RR targets Phospho-PKA C (Thr198) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | protein kinase, cAMP-dependent, catalytic, alpha |
| Calculated Molecular Weight | 41 kDa |
| Observed Molecular Weight | 38-43 kDa |
| GenBank Accession Number | NM_002730 |
| Gene Symbol | PRKACA |
| Gene ID (NCBI) | 5566 |
| RRID | AB_3745487 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P17612 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Protein kinase A (PKA) is a 3′-5′-cyclic adenosine monophosphate (cAMP)-dependent kinase that is at the center of diverse biological functions in numerous systems. Inactive PKA is composed of two catalytic (PKA-C) and two regulatory (PKA-R) subunits. The binding of cAMP to the PKA-R subunits activates the enzyme, causing the release of PKA-C. Humans have three genes encoding PKA-C: PRKACA, PRKACB, and PRKACG, and two types of PKA-R's: type PKA-RI (PKA-Riα and PKA-Riβ) and type PKA-RII (PKA-RIIα and PKA-RIIβ). In a direct side-by-side assay, λ-phosphatase nearly completely removed the phosphate from Akt1 Thr308, yet dephosphorylated PKAc at activation-loop Thr197 only marginally. Shielding of this phosphate by the kinase's own N- and C-terminal regions makes Thr197 behave as a constitutive maturation mark rather than a stimulus-regulated switch. (PMID: 36431013, PMID: 39592424, PMID: 31964716)
This antibody recognizes the phosphorylation at the Thr198 site of PKA-C (including PRKACA, PRKACB, and PRKACG). After removing methionine, the site is also called Thr197.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Phospho-PKA C (Thr198) antibody 87365-3-RR | Download protocol |
| IF protocol for Phospho-PKA C (Thr198) antibody 87365-3-RR | Download protocol |
| WB protocol for Phospho-PKA C (Thr198) antibody 87365-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Phospho-PKA C (Thr198) Recombinant monoclonal antibody (87365-3-RR) has 3 total citations. They appear in Phytomedicine, International Immunopharmacology, and Purinergic Signaling. Research fields include microbiota-gut-metabolite regulation in spleen deficiency syndrome, cAMP-PKA signaling in melatonin-mediated estradiol synthesis in the ovary, and cAMP/PKA/CREB pathway activation in cervical cancer cell and organoid proliferation.
| Species | Application | Title |
|---|---|---|
Phytomedicine Differential and complementary effects of Baizhu and Fuling on spleen deficiency syndrome by regulating microbiota-gut-metabolite axis. | ||
Int Immunopharmacol Melatonin orchestrates cAMP-PKA signaling to alleviate zearalenone-impaired the estradiol synthesis in mammal ovary. | ||
Purinergic Signal Targeting P2Y(14)R inhibits proliferation of cervical cancer cells and organoids and activates cAMP/PKA/CREB signaling pathway. |
