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Phospho-TAU (Thr217) Recombinant monoclonal antibody (87428-1-RR)

Phospho-TAU (Thr217) Recombinant monoclonal antibody

Phospho-TAU (Thr217) Uni-rAb^® Recombinant Antibody for WB, IHC, Dot Blot, ELISA

Cat No. 87428-1-RR

Clone No. 252442E8

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Dot Blot, ELISA

Synonyms

p Tau, p Tau (Thr217), p Tau Thr217, phos TAU, Phospho Tau

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

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-/ -

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Filter: AllWBIHCDot Blot

WB analysis using 87428-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 87428-1-RR (Phospho-TAU(Thr217) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human brain（AD） using 87428-1-RR
Immunohistochemical analysis of paraffin-embedded Normal human brain tissue (left) and human Alzheimer's disease brain(AD) slide (right) using 87428-1-RR (Phospho-TAU (Thr217) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human large intestine using 87428-1-RR
Immunohistochemical analysis of paraffin-embedded human large intestine tissue slide using 87428-1-RR (Phospho-TAU(Thr217) antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human large intestine using 87428-1-RR
Immunohistochemical analysis of paraffin-embedded human large intestine tissue slide using 87428-1-RR (Phospho-TAU(Thr217) antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Dot Blot experiment of / using 87428-1-RR
Dot blot analysis was used to confirm the specificity of 87428-1-RR,Phospho-TAU (Thr217) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (25ng) spotted is indicated next to each row.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	mouse blood
Positive IHC detected in	human brain (AD), human large intestine tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive Dot Blot detected in	/ /

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
DOT BLOT	DOT BLOT : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87428-1-RR targets Phospho-TAU (Thr217) in WB, IHC, Dot Blot, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	microtubule-associated protein tau
Calculated Molecular Weight	37-46, 79-81 kDa
Observed Molecular Weight	60-80 kDa
GenBank Accession Number	BC000558
Gene Symbol	TAU
Gene ID (NCBI)	4137
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P10636
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tau plays a crucial role in promoting and stabilizing microtubule assembly within neuronal axons. Phospho-TAU(Thr217) has emerged as a highly specific early biomarker for Alzheimer's disease (AD), detectable in both CSF and blood, effectively distinguishing AD from other dementias. This site is primarily phosphorylated by CDK5 and GSK-3β. Beyond its diagnostic value, p-T217 hyperphosphorylation is directly linked to synaptic dysfunction, neuronal loss, and cognitive decline in AD pathogenesis.