Phospho-TAU (Thr217) Recombinant monoclonal antibody, PBS Only

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• Phospho-TAU (Thr217) Recombinant monoclonal antibody, PBS Only (87428-1-PBS)

Phospho-TAU (Thr217) Uni-rAb^® Recombinant Antibody for WB, IHC, Dot Blot, Indirect ELISA

Cat No. 87428-1-PBS Copied!

Clone No. 252442E8

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Dot Blot, Indirect ELISA

Synonyms

p Tau, p Tau (Thr217), p Tau Thr217, phos TAU, Phospho Tau

• 

  WB analysis using 87428-1-RR (same clone as 87428-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87428-1-RR (Phospho-TAU(Thr217) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87428-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human brain（AD） using 87428-1-RR (same clone as 87428-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Normal human brain tissue (left) and human Alzheimer's disease brain(AD) slide (right) using 87428-1-RR (Phospho-TAU (Thr217) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87428-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human large intestine using 87428-1-RR (same clone as 87428-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human large intestine tissue slide using 87428-1-RR (Phospho-TAU(Thr217) antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87428-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human large intestine using 87428-1-RR (same clone as 87428-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human large intestine tissue slide using 87428-1-RR (Phospho-TAU(Thr217) antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87428-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Dot Blot experiment of / using 87428-1-RR (same clone as 87428-1-PBS)

  Dot blot analysis was used to confirm the specificity of 87428-1-RR,Phospho-TAU (Thr217) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (25ng) spotted is indicated next to each row. This data was developed using the same antibody clone with 87428-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

87428-1-PBS targets Phospho-TAU (Thr217) in WB, IHC, Dot Blot, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: microtubule-associated protein tau
• Calculated Molecular Weight: 37-46, 79-81 kDa
• Observed Molecular Weight: 60-80 kDa
• GenBank Accession Number: BC000558
• Gene Symbol: TAU
• Gene ID (NCBI): 4137
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P10636
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Tau plays a crucial role in promoting and stabilizing microtubule assembly within neuronal axons. Phospho-TAU(Thr217) has emerged as a highly specific early biomarker for Alzheimer's disease (AD), detectable in both CSF and blood, effectively distinguishing AD from other dementias. This site is primarily phosphorylated by CDK5 and GSK-3β. Beyond its diagnostic value, p-T217 hyperphosphorylation is directly linked to synaptic dysfunction, neuronal loss, and cognitive decline in AD pathogenesis.