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Fibrillarin Recombinant monoclonal antibody (87432-2-RR)

Fibrillarin Recombinant monoclonal antibody

Fibrillarin Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 87432-2-RR

Clone No.252802F8

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

FBL, EC:2.1.1.-, FIB, FIB1, FLRN

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

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Proteintech Guarantee

Filter: AllWBIF/ICC

WB analysis using 87432-2-RR
Various lysates were subjected to SDS PAGE followed by western blot with 87432-2-RR (FBL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 87432-2-RR
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Fibrillarin antibody (87432-2-RR, Clone: 252802F8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of NIH/3T3 using 87432-2-RR
Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Fibrillarin antibody (87432-2-RR, Clone: 252802F8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	A375 cells, HeLa cells, HepG2 cells, Jurkat cells, MOLT-4 cells, NIH/3T3 cells
Positive IF/ICC detected in	HepG2 cells, NIH/3T3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunofluorescence (IF)/ICC	IF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87432-2-RR targets Fibrillarin in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag8841 Product name: Recombinant human FBL protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-321 aa of BC019260 Sequence: MKPGFSPRGGGFGGRGGFGDRGGRGGRGGFGGGRGRGGGFRGRGRGGGGGGGGGGGGGRGGGGFHSGGNRGRGRGGKRGNQSGKNVMVEPHRHEGVFICRGKEDALVTKNLVPGESVYGEKRVSISEGDDKIEYRAWNPFRSKLAAAILGGVDQIHIKPGAKVLYLGAASGTTVSHVSDIVGPDGLVYAVEFSHRSGRDLINLAKKRTNIIPVIEDARHPHKYRMLIAMVDVIFADVAQPDQTRIVALNAHTFLRNGGHFVISIKANCIDSTASAEAVFASEVKKMQQENMKPQEQLTLEPYERDHAVVVGVYRPPPKVKN Predict reactive species
Full Name	fibrillarin
Calculated Molecular Weight	321 aa, 34 kDa
Observed Molecular Weight	34 kDa
GenBank Accession Number	BC019260
Gene Symbol	FBL
Gene ID (NCBI)	2091
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P22087
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fibrillarin (Fbl) is an essential nucleolar protein that plays an essential role in ribosome biogenesis and more particularly in the methylation of ribosomal RNAs and rDNA histones. FBL is highly expressed in various cancers and correlates with poor survival outcomes in cancer patients,such as hepatocellular carcinoma(PMID: 33376525). Knockdown of FBL sensitizes tumor cells and xenografts to DNA crosslinking agents, and leads to homologous recombination-mediated DNA repair defects(PMID: 37489617).