Fibrillarin Recombinant monoclonal antibody, PBS Only

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• Fibrillarin Recombinant monoclonal antibody, PBS Only (87432-2-PBS)

Fibrillarin Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 87432-2-PBS

Clone No.252802F8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

FBL, EC:2.1.1.-, FIB, FIB1, FLRN

• 

  WB analysis using 87432-2-RR (same clone as 87432-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87432-2-RR (FBL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87432-2-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 87432-2-RR (same clone as 87432-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Fibrillarin antibody (87432-2-RR, Clone: 252802F8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87432-2-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of NIH/3T3 using 87432-2-RR (same clone as 87432-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Fibrillarin antibody (87432-2-RR, Clone: 252802F8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87432-2-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

87432-2-PBS targets Fibrillarin in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag8841

  Product name: Recombinant human FBL protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-321 aa of BC019260

  Sequence: MKPGFSPRGGGFGGRGGFGDRGGRGGRGGFGGGRGRGGGFRGRGRGGGGGGGGGGGGGRGGGGFHSGGNRGRGRGGKRGNQSGKNVMVEPHRHEGVFICRGKEDALVTKNLVPGESVYGEKRVSISEGDDKIEYRAWNPFRSKLAAAILGGVDQIHIKPGAKVLYLGAASGTTVSHVSDIVGPDGLVYAVEFSHRSGRDLINLAKKRTNIIPVIEDARHPHKYRMLIAMVDVIFADVAQPDQTRIVALNAHTFLRNGGHFVISIKANCIDSTASAEAVFASEVKKMQQENMKPQEQLTLEPYERDHAVVVGVYRPPPKVKN

• Full Name: fibrillarin
• Calculated Molecular Weight: 321 aa, 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC019260
• Gene Symbol: FBL
• Gene ID (NCBI): 2091
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P22087
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Fibrillarin (Fbl) is an essential nucleolar protein that plays an essential role in ribosome biogenesis and more particularly in the methylation of ribosomal RNAs and rDNA histones. FBL is highly expressed in various cancers and correlates with poor survival outcomes in cancer patients,such as hepatocellular carcinoma(PMID: 33376525). Knockdown of FBL sensitizes tumor cells and xenografts to DNA crosslinking agents, and leads to homologous recombination-mediated DNA repair defects(PMID: 37489617).