Tested Applications
| Positive WB detected in | HT-29 cells, Caco-2 cells, T-47D cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87453-1-RR targets RAB25 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg5536 Product name: Recombinant human RAB25 protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 1-210 aa of NM_020387.3 Sequence: MGNGTEEDYNFVFKVVLIGESGVGKTNLLSRFTRNEFSHDSRTTIGVEFSTRTVMLGTAAVKAQIWDTAGLERYRAITSAYYRGAVGALLVFDLTKHQTYAVVERWLKELYDHAEATIVVMLVGNKSDLSQAREVPTEEARMFAENNGLLFLETSALDSTNVELAFETVLKEIFAKVSKQRQNSIRTNAITLGSAQAGQEPGPGEKRACC Predict reactive species |
| Full Name | RAB25, member RAS oncogene family |
| Calculated Molecular Weight | 23 kDa |
| Observed Molecular Weight | 24 kDa |
| GenBank Accession Number | NM_020387.3 |
| Gene Symbol | RAB25 |
| Gene ID (NCBI) | 57111 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P57735 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RAB25 (RAB25, member RAS oncogene family), also known as RAB11C or CATX-8, is a small GTPase belonging to the RAS superfamily of proteins. RAB25 is a key regulator of receptor recycling and polarized vesicular trafficking in epithelial cells. Unlike many RAB proteins, RAB25 expression is predominantly restricted to epithelial tissues and is frequently dysregulated in carcinomas. It functions as a critical determinant of cellular fate through its context-dependent roles in modulating major signaling pathways, including integrin recycling, growth factor receptor trafficking (e.g., EGFR, IGF1R), and autophagy.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RAB25 antibody 87453-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
