RAB25 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 87453-1-RR (same clone as 87453-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 87453-1-RR (RAB25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87453-1-PBS in a different storage buffer formulation.

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  Affinity of 87453-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87453-1-RR against Human RAB25 were performed. The affinity constant is 7.07 nM.

RAB25 Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 87453-1-PBS

Clone No.252713E9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

CATX 8, CATX8, CATX-8, Ras related protein Rab 25, Ras-related protein Rab-25

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Product Information

87453-1-PBS targets RAB25 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg5536

  Product name: Recombinant human RAB25 protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 1-210 aa of NM_020387.3

  Sequence: MGNGTEEDYNFVFKVVLIGESGVGKTNLLSRFTRNEFSHDSRTTIGVEFSTRTVMLGTAAVKAQIWDTAGLERYRAITSAYYRGAVGALLVFDLTKHQTYAVVERWLKELYDHAEATIVVMLVGNKSDLSQAREVPTEEARMFAENNGLLFLETSALDSTNVELAFETVLKEIFAKVSKQRQNSIRTNAITLGSAQAGQEPGPGEKRACC

• Full Name: RAB25, member RAS oncogene family
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: NM_020387.3
• Gene Symbol: RAB25
• Gene ID (NCBI): 57111
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P57735
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RAB25 (RAB25, member RAS oncogene family), also known as RAB11C or CATX-8, is a small GTPase belonging to the RAS superfamily of proteins. RAB25 is a key regulator of receptor recycling and polarized vesicular trafficking in epithelial cells. Unlike many RAB proteins, RAB25 expression is predominantly restricted to epithelial tissues and is frequently dysregulated in carcinomas. It functions as a critical determinant of cellular fate through its context-dependent roles in modulating major signaling pathways, including integrin recycling, growth factor receptor trafficking (e.g., EGFR, IGF1R), and autophagy.