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Tested Applications
| Positive WB detected in | HepG2 cells |
This antibody also recognizes the phosphorylation of RPS6KA1 at Ser221.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87534-1-RR targets Phospho-RPS6KA3 (Ser227) in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | ribosomal protein S6 kinase, 90kDa, polypeptide 3 |
| Calculated Molecular Weight | 740 aa, 84 kDa |
| Observed Molecular Weight | 84 kDa |
| GenBank Accession Number | BC096301 |
| Gene Symbol | RSK2 |
| Gene ID (NCBI) | 6197 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P51812 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Ribosomal S6 Kinase 2 (RSK2), a member of the p90RSK family of serine/threonine kinases, is known to be phosphorylated at Ser227 and Th577 in response to Adriamycin-induced DNA damage. This phosphorylation promotes RSK2 nuclear translocation and enhances RSK2 and Atm interactions in the nuclear fraction, playing an important role in the DNA damage pathway that maintains genomic stability by mediating cell cycle progression and DNA repair.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-RPS6KA3 (Ser227) antibody 87534-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
