Phospho-RPS6KA3 (Ser227) Recombinant monoclonal antibody, PBS Only

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  WB analysis of HepG2 using 87534-1-RR (same clone as 87534-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 87534-1-RR (Phospho-RPS6KA3 (Ser227) antibody) blocked with BSA only, Phospho-RPS6KA3 (Ser227) peptide or non-Phospho peptide at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87534-1-PBS in a different storage buffer formulation.

Phospho-RPS6KA3 (Ser227) Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 87534-1-PBS

Clone No.252865C9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

p90RSK3 (phospho Ser227), Phospho RSK2 (Ser227), Phospho-RPS6KA3, Phospho-RSK2, Phospho-RSK2 (Ser227)

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Product Information

87534-1-PBS targets Phospho-RPS6KA3 (Ser227) in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: ribosomal protein S6 kinase, 90kDa, polypeptide 3
• Calculated Molecular Weight: 740 aa, 84 kDa
• Observed Molecular Weight: 84 kDa
• GenBank Accession Number: BC096301
• Gene Symbol: RSK2
• Gene ID (NCBI): 6197
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P51812
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Ribosomal S6 Kinase 2 (RSK2), a member of the p90RSK family of serine/threonine kinases, is known to be phosphorylated at Ser227 and Th577 in response to Adriamycin-induced DNA damage. This phosphorylation promotes RSK2 nuclear translocation and enhances RSK2 and Atm interactions in the nuclear fraction, playing an important role in the DNA damage pathway that maintains genomic stability by mediating cell cycle progression and DNA repair.