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Sandwich ELISA sample test of MP03086-1
The mean EPHA2 concentration was determined to be 233.1 ng/mL in PC-3 cell extract based on a 1.5 mg/mL extract load and 4.0 ng/mL in HEK-293T cell extract based on a 1.2 mg/mL extract load.
Sandwich ELISA sample test of MP03086-1
Plasma of six individual healthy human donors was measured. The EPHA2 concentration of detected samples was determined to be 322.9 pg/mL with a range of 135.9-567.2 pg/mL.
Sandwich ELISA standard curve of MP03086-1
Sandwich ELISA standard curve of MP03086-1, Human EPHA2 Recombinant Matched Antibody Pair - PBS only. 87562-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0146. 87562-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-2500 pg/mL.
Cytometric bead array sample test of MP03086-1
The mean EPHA2 concentration was determined to be 65.4 ng/mL in PC-3 cell extract based on a 1.2 mg/mL extract load.
Cytometric bead array standard curve of MP03086-1
Cytometric bead array standard curve of MP03086-1, EPHA2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 87562-3-PBS. Detection antibody: 87562-2-PBS. Standard: Eg0146. Range: 0.156-20 ng/mL.
Affinity of 87562-2-PBS
Biolayer interferometry (BLl) kinetic assay of 87562-2-PBS against Human EPHA2 was performed. The affinity constant is 6.19 nM.

EPHA2 Recombinant monoclonal antibody, PBS Only (Detector)

Name: EPHA2 Recombinant monoclonal antibody, PBS Only (Detector)
Brand: Proteintech
SKU: 87562-2-PBS

EPHA2 Uni-rAb^® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA

Cat No. 87562-2-PBS

Clone No.252900G3

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

EC:2.7.10.1, ECK, EPH receptor A2, Ephrin type A receptor 2, Ephrin type-A receptor 2

Formulation: PBS Only

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

87562-2-PBS targets EPHA2 as part of a matched antibody pair:

MP03086-1: 87562-3-PBS capture and 87562-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg0146 Product name: Recombinant human EPHA2 protein Source: mammalian cells-derived, pHZ-KIsec Tag: Myc & 6*His Domain: 24-534 aa of BC037166 Sequence: AQGKEVVLLDFAAAGGELGWLTHPYGKGWDLMQNIMNDMPIYMYSVCNVMSGDQDNWLRTNWVYRGEAERIFIELKFTVRDCNSFPGGASSCKETFNLYYAESDLDYGTNFQKRLFTKIDTIAPDEITVSSDFEARHVKLNVEERSVGPLTRKGFYLAFQDIGACVALLSVRVYYKKCPELLQGLAHFPETIAGSDAPSLATVAGTCVDHAVVPPGGEEPRMHCAVDGEWLVPIGQCLCQAGYEKVEDACQACSPGFFKFEASESPCLECPEHTLPSPEGATSCECEEGFFRAPQDPASMPCTRPPSAPHYLTAVGMGAKVELRWTPPQDSGGREDIVYSVTCEQCWPESGECGPCEASVRYSEPPHGLTRTSVTVSDLEPHMNYTFTVEARNGVSGLVTSRSFRTASVSINQTEPPKVRLEGRSTTSLSVSWSIPPPQQSRVWKYEVTYRKKGDSNSYNVRRTEGFSVTLDDLAPDTTYLVQVQALTQEGQGAGSKVHEFQTLSPEGSGN Predict reactive species
Full Name	EPH receptor A2
Calculated Molecular Weight	976 aa, 108 kDa
GenBank Accession Number	BC037166
Gene Symbol	EPHA2
Gene ID (NCBI)	1969
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P29317
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.