Home
Products
Primary Antibodies
Recombinant Antibodies
IFN-alpha Recombinant monoclonal antibody (87610-3-RR)

IFN-alpha Recombinant monoclonal antibody

IFN-alpha Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 87610-3-RR

Clone No.252039C10

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

IFN ALPHA, IFNA1, IFN alpha 1/13, IFN alpha1, IFNA 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Filter: AllWBIF/ICCAffinity

WB analysis of Recombinant protein using 87610-3-RR
Human IFN-alpha recombinant protein was subjected to SDS PAGE followed by western blot with 87610-3-RR (IFNA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 87610-3-RR
CHO cells transfected with human IFN-alpha were subjected to SDS PAGE followed by western blot with 87610-3-RR (IFNA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of Transfected CHO using 87610-3-RR
Immunofluorescent analysis of (4% PFA) fixed Transfected CHO using IFN-alpha antibody (87610-3-RR, Clone: 252039C10 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Affinity of 87610-3-RR
Biolayer interferometry (BLI) kinetic assays of 87610-3-RR against Human IFN-alpha were performed. The affinity constant is 0.989 nM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	Recombinant protein, Transfected with IFNA1 CHO cells
Positive IF/ICC detected in	Transfected CHO

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunofluorescence (IF)/ICC	IF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87610-3-RR targets IFN-alpha in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg2592 Product name: Recombinant Human IFNA1 protein (rFc Tag)(HPLC verified) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 24-189 aa of NM_024013.2 Sequence: CDLPETHSLDNRRTLMLLAQMSRISPSSCLMDRHDFGFPQEEFDGNQFQKAPAISVLHELIQQIFNLFTTKDSSAAWDEDLLDKFCTELYQQLNDLEACVMQEERVGETPLMNADSILAVKKYFRRITLYLTEKKYSPCAWEVVRAEIMRSLSLSTNLQERLRRKE Predict reactive species
Full Name	IFNA1
Calculated Molecular Weight	22 kDa
GenBank Accession Number	NM_024013.2
Gene Symbol	IFN alpha1
Gene ID (NCBI)	3439
RRID	AB_3745647
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P01562
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IFNA1 (IF-alpha) is a member of the Type I IFN (1) family best known for their antiviral activity. It is a key cytokine regulating the activity of B cells, T-helper cells (Th cells), cDCs and natural killer cells (NK cells). IFNA1 induces B cell maturation into plasma cells and immunoglobulin production. IFNA1 plays an important role in the pathogenesis of systemic lupus erythematosus (SLE). IFNA1 was the first cytokine to show clinical benefit in the treatment of certain types of cancer, including melanoma, chronic myelogenous leukemia, and renal cancer.