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WB analysis using 87612-1-RR (same clone as 87612-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 87612-1-RR (CNTN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87612-1-PBS in a different storage buffer formulation.
Sandwich ELISA sample test of MP03227-1
The mean CNTN1 concentration was determined to be 429.57 ng/mL in A549 cell extract based on a 1.20 mg/mL extract load and 191.70 ng/mL in HT-29 cell extract based on a 1.10 mg/mL extract load.
Sandwich ELISA standard curve of MP03227-1
Sandwich ELISA standard curve of MP03227-1, Human CNTN1 Recombinant Matched Antibody Pair - PBS only. 87612-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg6555. 87612-1-PBS was HRP conjugated as the detection antibody. Range: 7.81-500 ng/mL.
IP experiment of mouse brain using 87612-1-RR (same clone as 87612-1-PBS)
IP result of anti-CNTN1 (IP:87612-1-RR, 4 ug; Detection: 87612-1-RR 1:5000) with mouse brain tissue lysate 1680 ug. This data was developed using the same antibody clone with 87612-1-PBS in a different storage buffer formulation.
IP experiment of rat brain using 87612-1-RR (same clone as 87612-1-PBS)
IP result of anti-CNTN1 (IP:87612-1-RR, 4 ug; Detection: 87612-1-RR 1:5000) with rat brain tissue lysate 1720 ug. This data was developed using the same antibody clone with 87612-1-PBS in a different storage buffer formulation.
Affinity of 87612-1-RR
Biolayer interferometry (BLl) kinetic assays of 87612-1-RR against Human CNTN1 were performed. The affinity constant is 1.72 nM.

CNTN1 Recombinant monoclonal antibody, PBS Only (Detector)

Name: CNTN1 Recombinant monoclonal antibody, PBS Only (Detector)
Brand: Proteintech
SKU: 87612-1-PBS

CNTN1 Uni-rAb^® Recombinant Antibody for WB, IP, Sandwich ELISA, Indirect ELISA

Cat No. 87612-1-PBS

Clone No.252906D4

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Sandwich ELISA, Indirect ELISA

Synonyms

contactin 1, Contactin-1, F3, Glycoprotein gp135, GP135

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

87612-1-PBS targets CNTN1 as part of a matched antibody pair:

MP03227-1: 87612-2-PBS capture and 87612-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg6555 Product name: Recombinant human CNTN1 protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 21-993 aa of NM_001843.3 Sequence: EFTWYRRYGHGVSEEDKGFGPIFEEQPINTIYPEESLEGKVSLNCRARASPFPVYKWRMNNGDVDLTSDRYSMVGGNLVINNPDKQKDAGIYYCLASNNYGMVRSTEATLSFGYLDPFPPEERPEVRVKEGKGMVLLCDPPYHFPDDLSYRWLLNEFPVFITMDKRRFVSQTNGNLYIANVEASDKGNYSCFVSSPSITKSVFSKFIPLIPIPERTTKPYPADIVVQFKDVYALMGQNVTLECFALGNPVPDIRWRKVLEPMPSTAEISTSGAVLKIFNIQLEDEGIYECEAENIRGKDKHQARIYVQAFPEWVEHINDTEVDIGSDLYWPCVATGKPIPTIRWLKNGYAYHKGELRLYDVTFENAGMYQCIAENTYGAIYANAELKILALAPTFEMNPMKKKILAAKGGRVIIECKPKAAPKPKFSWSKGTEWLVNSSRILIWEDGSLEINNITRNDGGIYTCFAENNRGKANSTGTLVITDPTRIILAPINADITVGENATMQCAASFDPALDLTFVWSFNGYVIDFNKENIHYQRNFMLDSNGELLIRNAQLKHAGRYTCTAQTIVDNSSASADLVVRGPPGPPGGLRIEDIRATSVALTWSRGSDNHSPISKYTIQTKTILSDDWKDAKTDPPIIEGNMEAARAVDLIPWMEYEFRVVATNTLGRGEPSIPSNRIKTDGAAPNVAPSDVGGGGGRNRELTITWAPLSREYHYGNNFGYIVAFKPFDGEEWKKVTVTNPDTGRYVHKDETMSPSTAFQVKVKAFNNKGDGPYSLVAVINSAQDAPSEAPTEVGVKVLSSSEISVHWEHVLEKIVESYQIRYWAAHDKEEAANRVQVTSQEYSARLENLLPDTQYFIEVGACNSAGCGPPSDMIEAFTKKAPPSQPPRIISSVRSGSRYIITWDHVVALSNESTVTGYKVLYRPDGQHDGKLYSTHKHSIEVPIPRDGEYVVEVRAHSDGGDGVVSQVKIS Predict reactive species
Full Name	contactin 1
Calculated Molecular Weight	113 kDa
Observed Molecular Weight	135 kDa
GenBank Accession Number	NM_001843.3
Gene Symbol	CNTN1
Gene ID (NCBI)	1272
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q12860
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Contactin-1 (CNTN1), also known as Glycoprotein gp135 or Neural cell surface protein F3, is a neuronal cell adhesion molecule of the immunoglobulin superfamily (PMID: 3049624). It consists of six Ig domains, four fibronectin-like motifs, and a glycosylphosphatidylinositol (GPI)-moiety. Expressed by the central and peripheral neurons, CNTN1 contributes to the formation and function of neuronal connections. It plays a role in axon extension and formation of septate-like junctions between axons and myelinating glial cells (PMID: 23724143). In addition, acting as a ligand for Notch, CNTN1 initiates a NOTCH/DTX1 signaling pathway that promotes oligodendrocyte maturation and myelination (PMID: 14567914).