Product Information
87643-2-PBS targets GTF2B as part of a matched antibody pair:
MP03104-1: 87643-2-PBS capture and 87643-1-PBS detection (validated in Cytometric bead array)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag9547 Product name: Recombinant human GTF2B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-316 aa of BC020597 Sequence: MASTSRLDALPRVTCPNHPDAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAASFDEFGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLILKALETSVDLITTGDFMSRFCSNLCLPKQVQMAATHIARKAVELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGDIAGVADVTIRQSYRLIYPRAPDLFPTDFKFDTPVDKLPQL Predict reactive species |
| Full Name | general transcription factor IIB |
| Calculated Molecular Weight | 316 aa, 35 kDa |
| Observed Molecular Weight | 30-35 kDa |
| GenBank Accession Number | BC020597 |
| Gene Symbol | GTF2B |
| Gene ID (NCBI) | 2959 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q00403 |
| Storage Buffer | PBS only, pH 7.3. |
| Storage Conditions | Store at -80°C. |
Background Information
GTF2B encodes transcription factor IIB (TFIIB), one of the ubiquitous general transcription factors essential for transcription initiation by RNA polymerase II (Pol II). Localizing to the nucleus, TFIIB forms a complex with transcription factors IID and IIA and serves as a critical bridge between TFIID-the factor that initially recognizes the promoter-and the Pol II machinery, thereby enabling pre-initiation complex (PIC) assembly and Pol II recruitment to promoter DNA. Beyond this core architectural role, TFIIB is subject to regulatory acetylation and autoacetylation, and emerging evidence links its dysregulation to pathological states, including potential contributions to proliferation in hepatocellular carcinoma and tumor phenotypes in growth hormone-secreting pituitary adenomas.
