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Phospho-Histone H3 (Ser28) Recombinant monoclonal antibody (87654-1-RR)

Phospho-Histone H3 (Ser28) Recombinant monoclonal antibody

Phospho-Histone H3 (Ser28) Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87654-1-RR

Clone No.250369D9

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

H3, Histone H3 (Ser28), p Histone H3, p Histone H3 (Ser28), p Histone H3 Ser28

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

- +

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WB analysis using 87654-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 87654-1-RR (Phospho-Histone H3 (Ser28) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Alpha Tubulin Monoclonal antibody (66031-1-Ig) as loading control.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 87654-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 87654-1-RR (Phospho-Histone H3 (Ser28) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Alpha Tubulin Monoclonal antibody (66031-1-Ig) as loading control.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in

nocodazole treated HeLa cells, nocodazole treated NIH/3T3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87654-1-RR targets Phospho-Histone H3 (Ser28) in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	histone cluster 1, H3a
Observed Molecular Weight	17 kDa
GenBank Accession Number	BC066245
Gene Symbol	HIST1H3A
Gene ID (NCBI)	8350
RRID	AB_3745676
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P68431
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

H3S28ph and H3S10ph appear simultaneously in the early stages of mitosis, starting from prophase I (chromosome condensation), distributed along the chromosomes, and participating in chromosome condensation and separation. H3S28ph, as a marker of cell proliferation and transformation, has important value in tumor research.