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Phospho-Histone H3 (Ser28) Recombinant monoclonal antibody, PBS Only (87654-1-PBS)

Phospho-Histone H3 (Ser28) Recombinant monoclonal antibody, PBS Only

Name: Phospho-Histone H3 (Ser28) Recombinant monoclonal antibody, PBS Only, 100ug
Brand: Proteintech
SKU: 87654-1-PBS-100UG

Phospho-Histone H3 (Ser28) Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 87654-1-PBS

Clone No.250369D9

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

H3, Histone H3 (Ser28), p Histone H3, p Histone H3 (Ser28), p Histone H3 Ser28

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

SKU:

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Freight/Packing: -

Quantity

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Filter: AllWB

WB analysis using 87654-1-RR (same clone as 87654-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 87654-1-RR (Phospho-Histone H3 (Ser28) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Alpha Tubulin Monoclonal antibody (66031-1-Ig) as loading control. This data was developed using the same antibody clone with 87654-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
Various lysates were subjected to SDS PAGE followed by western blot with 87654-1-RR (Phospho-Histone H3 (Ser28) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Alpha Tubulin Monoclonal antibody (66031-1-Ig) as loading control. This data was developed using the same antibody clone with 87654-1-PBS in a different storage buffer formulation.
WB analysis using 87654-1-RR (same clone as 87654-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 87654-1-RR (Phospho-Histone H3 (Ser28) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Alpha Tubulin Monoclonal antibody (66031-1-Ig) as loading control. This data was developed using the same antibody clone with 87654-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
Various lysates were subjected to SDS PAGE followed by western blot with 87654-1-RR (Phospho-Histone H3 (Ser28) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Alpha Tubulin Monoclonal antibody (66031-1-Ig) as loading control. This data was developed using the same antibody clone with 87654-1-PBS in a different storage buffer formulation.

Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

87654-1-PBS targets Phospho-Histone H3 (Ser28) in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	histone cluster 1, H3a
Observed Molecular Weight	17 kDa
GenBank Accession Number	BC066245
Gene Symbol	HIST1H3A
Gene ID (NCBI)	8350
RRID	AB_3745676
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P68431
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

H3S28ph and H3S10ph appear simultaneously in the early stages of mitosis, starting from prophase I (chromosome condensation), distributed along the chromosomes, and participating in chromosome condensation and separation. H3S28ph, as a marker of cell proliferation and transformation, has important value in tumor research.