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RRAS Recombinant monoclonal antibody, PBS Only (Detector) (87667-1-PBS)

RRAS Recombinant monoclonal antibody, PBS Only (Detector)

Name: RRAS Recombinant monoclonal antibody, PBS Only (Detector), 100ug
Brand: Proteintech
SKU: 87667-1-PBS-100UG

RRAS Uni-rAb^® Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA

Cat No. 87667-1-PBS

Clone No.253028H2

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Sandwich ELISA, Indirect ELISA

Synonyms

EC:3.6.5.-, p23, Ras related protein R Ras, Ras-related protein R-Ras

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

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Filter: AllWBSandwich ELISA sample testSandwich ELISA

WB analysis using 87667-1-RR (same clone as 87667-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 87667-1-RR (RRAS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87667-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Sandwich ELISA sample test of MP03286-1
The mean RRAS concentration was determined to be 1,971.10 ng/mL in Jurkat cell extract based on a 1.20 mg/mL extract load, 926.11 ng/mL in U2OS cell extract based on a 1.20 mg/mL extract load, 1,001.10 ng/mL in HeLa cell extract based on a 1.20 mg/mL extract load, 1,377.33 ng/mL in BxPC-3 cell extract based on a 1.20 mg/mL extract load, 707.70 ng/mL in Raji cell extract based on a 1.20 mg/mL extract load and 670.37 ng/mL in HL-60 cell extract based on a 1.50 mg/mL extract load.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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The mean RRAS concentration was determined to be 1,971.10 ng/mL in Jurkat cell extract based on a 1.20 mg/mL extract load, 926.11 ng/mL in U2OS cell extract based on a 1.20 mg/mL extract load, 1,001.10 ng/mL in HeLa cell extract based on a 1.20 mg/mL extract load, 1,377.33 ng/mL in BxPC-3 cell extract based on a 1.20 mg/mL extract load, 707.70 ng/mL in Raji cell extract based on a 1.20 mg/mL extract load and 670.37 ng/mL in HL-60 cell extract based on a 1.50 mg/mL extract load.
Sandwich ELISA standard curve of MP03286-1
Sandwich ELISA standard curve of MP03286-1, Human RRAS Recombinant Matched Antibody Pair - PBS only. 87667-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg6612. 87667-1-PBS was HRP conjugated as the detection antibody. Range: 0.625-40 ng/mL.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

87667-1-PBS targets RRAS as part of a matched antibody pair:

MP03286-1: 87667-2-PBS capture and 87667-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg6612 Product name: Recombinant human RRAS protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 23-201 aa of NM_006270.5 Sequence: DPPPSETHKLVVVGGGGVGKSALTIQFIQSYFVSDYDPTIEDSYTKICSVDGIPARLDILDTAGQEEFGAMREQYMRAGHGFLLVFAINDRQSFNEVGKLFTQILRVKDRDDFPVVLVGNKADLESQRQVPRSEASAFGASHHVAYFEASAKLRLNVDEAFEQLVRAVRKYQEQELPPS Predict reactive species
Full Name	related RAS viral (r-ras) oncogene homolog
Calculated Molecular Weight	23 kDa
Observed Molecular Weight	23 kDa
GenBank Accession Number	NM_006270.5
Gene Symbol	RRAS
Gene ID (NCBI)	6237
RRID	AB_3745685
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P10301
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

RRAS belongs to the Ras subfamily of small G-proteins, which are frequently implicated in cell growth and differentiation.RRAS is a small GTPase involved in diverse processes including angiogenesis, vascular homeostasis and regeneration, cell adhesion, and neuronal axon guidance. Mutations in this gene are found in many invasive cancers.