Tested Applications
| Positive WB detected in | A431 cells, U-87 MG cells, SH-SY5Y cells, PC-3 cells, mouse brain tissue, rat brain tissue, mouse cerebellum tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87673-1-RR targets NCS1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg5439 Product name: Recombinant human NCS1 protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 1-190 aa of NM_014286.3 Sequence: MGKSNSKLKPEVVEELTRKTYFTEKEVQQWYKGFIKDCPSGQLDAAGFQKIYKQFFPFGDPTKFATFVFNVFDENKDGRIEFSEFIQALSVTSRGTLDEKLRWAFKLYDLDNDGYITRNEMLDIVDAIYQMVGNTVELPEEENTPEKRVDRIFAMMDKNADGKLTLQEFQEGSKADPSIVQALSLYDGLV Predict reactive species |
| Full Name | frequenin homolog (Drosophila) |
| Calculated Molecular Weight | 22 kDa |
| Observed Molecular Weight | 20 kDa |
| GenBank Accession Number | NM_014286.3 |
| Gene Symbol | NCS1 |
| Gene ID (NCBI) | 23413 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P62166-1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Neuronal Calcium Sensor 1 is a member of the NCS protein family, a group of evolutionarily conserved, calcium-binding proteins predominantly expressed in the nervous system and neuroendocrine cells. NCS-1 is the vertebrate homolog of the extensively studied Drosophila protein Frequenin. It plays a crucial and multifaceted role in regulating neuronal signaling, synaptic plasticity, and neurotransmitter release.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NCS1 antibody 87673-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
