NCS1 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 87673-1-RR (same clone as 87673-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87673-1-RR (NCS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87673-1-PBS in a different storage buffer formulation.

• 

  Affinity of 87673-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87673-1-RR against Human NCS1 were performed. The affinity constant is 1.58 nM.

NCS1 Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 87673-1-PBS

Clone No.253130F1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

FREQ, FLUP, Frequenin homolog, frequenin homolog (Drosophila), Frequenin like protein

---

Product Information

87673-1-PBS targets NCS1 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg5439

  Product name: Recombinant human NCS1 protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 1-190 aa of NM_014286.3

  Sequence: MGKSNSKLKPEVVEELTRKTYFTEKEVQQWYKGFIKDCPSGQLDAAGFQKIYKQFFPFGDPTKFATFVFNVFDENKDGRIEFSEFIQALSVTSRGTLDEKLRWAFKLYDLDNDGYITRNEMLDIVDAIYQMVGNTVELPEEENTPEKRVDRIFAMMDKNADGKLTLQEFQEGSKADPSIVQALSLYDGLV

• Full Name: frequenin homolog (Drosophila)
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: NM_014286.3
• Gene Symbol: NCS1
• Gene ID (NCBI): 23413
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P62166-1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Neuronal Calcium Sensor 1 is a member of the NCS protein family, a group of evolutionarily conserved, calcium-binding proteins predominantly expressed in the nervous system and neuroendocrine cells. NCS-1 is the vertebrate homolog of the extensively studied Drosophila protein Frequenin. It plays a crucial and multifaceted role in regulating neuronal signaling, synaptic plasticity, and neurotransmitter release.