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Phospho-EGFR (Tyr1069) Recombinant monoclonal antibody (87711-2-RR)

Phospho-EGFR (Tyr1069) Recombinant monoclonal antibody

Phospho-EGFR (Tyr1069) Uni-rAb^® Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 87711-2-RR

Clone No.253169E2

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

EGF Receptor (Tyr1045), EGFR (Tyr1045), EGFR (Tyr1069), p-EGF Receptor, p-EGF Receptor (Tyr1045)

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

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Freight/Packing: -

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Filter: AllWBPattern DiagramFC (Intra)

WB analysis using 87711-2-RR
Non-treated HeLa cells and EGF (HZ-1326) treated HeLa cells were subjected to SDS PAGE followed by western blot with 87711-2-RR (Phospho-EGFR (Tyr1069) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (86930-1-RR) antibody as a loading control.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 87711-2-RR
Non-treated A431 cells and EGF (HZ-1326) treated A431 cells were subjected to SDS PAGE followed by western blot with 87711-2-RR (Phospho-EGFR (Tyr1069) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (86930-1-RR) antibody as a loading control.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Schematic diagram of the phosphorylation sites of EGFR.
In the full-length versus signal-peptide-removed forms, nomenclature differs but the modification site is conserved.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of A431 using 87711-2-RR
1X10^6 A431 cells untreated (dashed lines) or treated with EGF which intracellularly stained with 0.13 ug Phospho-EGFR (Tyr1069) Recombinant monoclonal antibody (87711-2-RR, Clone:253169E2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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1X10^6 A431 cells untreated (dashed lines) or treated with EGF which intracellularly stained with 0.13 ug Phospho-EGFR (Tyr1069) Recombinant monoclonal antibody (87711-2-RR, Clone:253169E2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	EGF treated HeLa cells, EGF treated A431 cells
Positive FC (Intra) detected in	EGF treated A431 cells

This antibody, also known as EGFR Tyr1045, is the site where the signal peptide is removed.

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87711-2-RR targets Phospho-EGFR (Tyr1069) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Calculated Molecular Weight	1210 aa, 134 kDa
Observed Molecular Weight	175 kDa
GenBank Accession Number	BC094761
Gene Symbol	EGFR
Gene ID (NCBI)	1956
RRID	AB_3745710
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P00533
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Epidermal Growth Factor Receptor (EGFR) is one of the receptor tyrosine kinases (RTKs), members of the ErbB/HER family which consists of ErbB1 (EGFR or HER1), ErbB2 (HER2 or Neu), ErbB3 (HER3), and ErbB4 (HER4). Aberrant activation of EGFR is among the most common oncogenic driving events in human cancer, which is mediated largely through different classes of genomic alterations within the EGFR gene. Phosphorylation of EGFR Tyr1069 acts as the degron of EGFR and is recognized by c-Cbl, enhancing the ubiquitination and degradation of EGFR. EGFR stability is tightly regulated upon EGF stimulation. (PMID: 35164092, PMID: 29464683, PMID: 37085908). Some scientists call EGFR Tyr1069 as EGFR Tyr1045, the site where the signaling peptide is removed.

Protocols

Product Specific Protocols
FC protocol for Phospho-EGFR (Tyr1069) antibody 87711-2-RR	Download protocol
WB protocol for Phospho-EGFR (Tyr1069) antibody 87711-2-RR	Download protocol