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Phospho-EGFR (Tyr1069) Recombinant monoclonal antibody, PBS Only (87711-2-PBS)

Phospho-EGFR (Tyr1069) Recombinant monoclonal antibody, PBS Only

Name: Phospho-EGFR (Tyr1069) Recombinant monoclonal antibody, PBS Only, 100ug
Brand: Proteintech
SKU: 87711-2-PBS-100UG

Phospho-EGFR (Tyr1069) Uni-rAb^® Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Cat No. 87711-2-PBS

Clone No.253169E2

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

EGF Receptor (Tyr1045), EGFR (Tyr1045), EGFR (Tyr1069), p-EGF Receptor, p-EGF Receptor (Tyr1045)

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

SKU:

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Freight/Packing: -

Quantity

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Filter: AllWBPattern DiagramFC (Intra)

WB analysis using 87711-2-RR (same clone as 87711-2-PBS)
Non-treated HeLa cells and EGF (HZ-1326) treated HeLa cells were subjected to SDS PAGE followed by western blot with 87711-2-RR (Phospho-EGFR (Tyr1069) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (86930-1-RR) antibody as a loading control. This data was developed using the same antibody clone with 87711-2-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
Non-treated HeLa cells and EGF (HZ-1326) treated HeLa cells were subjected to SDS PAGE followed by western blot with 87711-2-RR (Phospho-EGFR (Tyr1069) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (86930-1-RR) antibody as a loading control. This data was developed using the same antibody clone with 87711-2-PBS in a different storage buffer formulation.
WB analysis using 87711-2-RR (same clone as 87711-2-PBS)
Non-treated A431 cells and EGF (HZ-1326) treated A431 cells were subjected to SDS PAGE followed by western blot with 87711-2-RR (Phospho-EGFR (Tyr1069) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (86930-1-RR) antibody as a loading control. This data was developed using the same antibody clone with 87711-2-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
Non-treated A431 cells and EGF (HZ-1326) treated A431 cells were subjected to SDS PAGE followed by western blot with 87711-2-RR (Phospho-EGFR (Tyr1069) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (86930-1-RR) antibody as a loading control. This data was developed using the same antibody clone with 87711-2-PBS in a different storage buffer formulation.
Schematic diagram of the phosphorylation sites of EGFR.
In the full-length versus signal-peptide-removed forms, nomenclature differs but the modification site is conserved.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of A431 using 87711-2-RR (same clone as 87711-2-PBS)
1X10^6 A431 cells untreated (dashed lines) or treated with EGF which intracellularly stained with 0.13 ug Phospho-EGFR (Tyr1069) Recombinant monoclonal antibody (87711-2-RR, Clone:253169E2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026). This data was developed using the same antibody clone with 87711-2-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1X10^6 A431 cells untreated (dashed lines) or treated with EGF which intracellularly stained with 0.13 ug Phospho-EGFR (Tyr1069) Recombinant monoclonal antibody (87711-2-RR, Clone:253169E2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026). This data was developed using the same antibody clone with 87711-2-PBS in a different storage buffer formulation.

Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

87711-2-PBS targets Phospho-EGFR (Tyr1069) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Calculated Molecular Weight	1210 aa, 134 kDa
Observed Molecular Weight	175 kDa
GenBank Accession Number	BC094761
Gene Symbol	EGFR
Gene ID (NCBI)	1956
RRID	AB_3745710
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P00533
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Epidermal Growth Factor Receptor (EGFR) is one of the receptor tyrosine kinases (RTKs), members of the ErbB/HER family which consists of ErbB1 (EGFR or HER1), ErbB2 (HER2 or Neu), ErbB3 (HER3), and ErbB4 (HER4). Aberrant activation of EGFR is among the most common oncogenic driving events in human cancer, which is mediated largely through different classes of genomic alterations within the EGFR gene. Phosphorylation of EGFR Tyr1069 acts as the degron of EGFR and is recognized by c-Cbl, enhancing the ubiquitination and degradation of EGFR. EGFR stability is tightly regulated upon EGF stimulation. (PMID: 35164092, PMID: 29464683, PMID: 37085908). Some scientists call EGFR Tyr1069 as EGFR Tyr1045, the site where the signaling peptide is removed.