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IL-24 Recombinant monoclonal antibody (87821-1-RR)

IL-24 Recombinant monoclonal antibody

IL-24 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 87821-1-RR

Clone No.251947B5

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

IL24, IL 24, interleukin 24, Interleukin-24, Melanoma differentiation-associated gene 7 protein

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

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Filter: AllWBIF/ICCAffinity

WB analysis of CHO using 87821-1-RR
CHO cells transfected with human IL-24 were subjected to SDS PAGE followed by western blot with 87821-1-RR (IL-24 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of Jurkat using 87821-1-RR
Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using IL-24 antibody (87821-1-RR, Clone: 251947B5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Affinity of 87821-1-RR
Biolayer interferometry (BLI) kinetic assays of 87821-1-RR against Human IL-24 were performed. The affinity constant is below 1 pM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	Transfected with human IL-24 CHO cells
Positive IF/ICC detected in	Jurkat cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87821-1-RR targets IL-24 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg4971 Product name: Recombinant Human IL-24 protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 52-206 aa of BC009681 Sequence: QEFHFGPCQVKGVVPQKLWEAFWAVKDTMQAQDNITSARLLQQEVLQNVSDAESCYLVHTLLEFYLKTVFKNYHNRTVEVRTLKSFSTLANNFVLIVSQLQPSQENEMFSIRDSAHRRFLLFRRAFKQLDVEAALTKALGEVDILLTWMQKFYKL Predict reactive species
Full Name	interleukin 24
Calculated Molecular Weight	207 aa, 24 kDa
GenBank Accession Number	BC009681
Gene Symbol	IL-24
Gene ID (NCBI)	11009
RRID	AB_3745770
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q13007
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-24 (IL-24) , also known as Melanoma Differentiation-Associated gene 7 (mda-7) , is a protein that belongs to the IL-10 family of cytokines. It was initially discovered in 1995 by researchers Paul B. Fisher and colleagues as a gene that is induced during the terminal differentiation of melanoma cells. Unlike many other cytokines, IL-24 is secreted primarily by activated monocytes, macrophages, and T helper 2 (Th2) cells.