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TNFRSF6B Recombinant monoclonal antibody (87840-1-RR)

TNFRSF6B Recombinant monoclonal antibody

TNFRSF6B Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87840-1-RR

Clone No.253457A6

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

DcR3, Decoy receptor 3, Decoy receptor for Fas ligand, M68, TR6

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

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Filter: AllWBAffinity

WB analysis using 87840-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 87840-1-RR (TNFRSF6B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of ACHN using 87840-1-RR
ACHN cells were subjected to SDS PAGE followed by western blot with 87840-1-RR (TNFRSF6B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Affinity of 87840-1-RR
Biolayer interferometry (BLI) kinetic assays of 87840-1-RR against Mouse TNFRSF6B were performed. The affinity constant is 0.131 nM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in

ACHN cells, SW480 cells, HCT 116 cells, SW 1990 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87840-1-RR targets TNFRSF6B in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg3110 Product name: Recombinant human TNFRSF6B protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 30-300 aa of NM_003823.3 Sequence: VAETPTYPWRDAETGERLVCAQCPPGTFVQRPCRRDSPTTCGPCPPRHYTQFWNYLERCRYCNVLCGEREEEARACHATHNRACRCRTGFFAHAGFCLEHASCPPGAGVIAPGTPSQNTQCQPCPPGTFSASSSSSEQCQPHRNCTALGLALNVPGSSSHDTLCTSCTGFPLSTRVPGAEECERAVIDFVAFQDISIKRLQRLLQALEAPEGWGPTPRAGRAALQLKLRRRLTELLGAQDGALLVRLLQALRVARMPGLERSVRERFLPVH Predict reactive species
Full Name	tumor necrosis factor receptor superfamily, member 6b, decoy
Calculated Molecular Weight	33 kDa
Observed Molecular Weight	35 kDa
GenBank Accession Number	NM_003823.3
Gene Symbol	TNFRSF6B
Gene ID (NCBI)	8771
RRID	AB_3745781
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	O95407
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The tumor necrosis factor receptor super-family member 6b (TNFRSF6B) is over-expressed in various human cancers. TNFRSF6B mRNA and protein expression in the tumor tissues negatively correlated with apoptosis. Cell proliferation was decreased, cell cycle was arrested in G1/S-phase, apoptosis was increased, and migration ability was inhibited by anti-TNFRSF6B McAb in vitro. Anti-TNFRSF6B McAb could be useful to suppress proliferation and induce apoptosis in HCC. Thus, TNFRSF6B might be a critical, targeted therapy strategy for HCC (PMID: 20591579).