TNFRSF6B Recombinant monoclonal antibody, PBS Only (Detector)

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• TNFRSF6B Recombinant monoclonal antibody, PBS Only (Detector) (87840-1-PBS)

TNFRSF6B Uni-rAb^® Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA

Cat No. 87840-1-PBS

Clone No.253457A6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Sandwich ELISA, Indirect ELISA

Synonyms

DcR3, Decoy receptor 3, Decoy receptor for Fas ligand, M68, TR6

• 

  WB analysis using 87840-1-RR (same clone as 87840-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87840-1-RR (TNFRSF6B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87840-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of ACHN using 87840-1-RR (same clone as 87840-1-PBS)

  ACHN cells were subjected to SDS PAGE followed by western blot with 87840-1-RR (TNFRSF6B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87840-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Sandwich ELISA sample test of MP03265-1

  SW 480 cells were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human DcR3/TNFRSF6B, and measured 29.05 ng/mL.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Sandwich ELISA sample test of MP03265-1

  Plasma of five individual healthy human donors was measured. The human DcR3/TNFRSF6B concentration of detected samples was determined to be 2.47 ng/mL with a range of 0.38 - 9.39 ng/mL.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Sandwich ELISA standard curve of MP03265-1

  Sandwich ELISA standard curve of MP03265-1, Human DcR3/TNFRSF6B Recombinant Matched Antibody Pair - PBS only. 87840-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg3110. 87840-1-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity of 87840-1-RR

  Biolayer interferometry (BLI) kinetic assays of 87840-1-RR against Mouse TNFRSF6B were performed. The affinity constant is 0.131 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

87840-1-PBS targets TNFRSF6B as part of a matched antibody pair:

MP03265-1: 87840-2-PBS capture and 87840-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg3110

  Product name: Recombinant human TNFRSF6B protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 30-300 aa of NM_003823.3

  Sequence: VAETPTYPWRDAETGERLVCAQCPPGTFVQRPCRRDSPTTCGPCPPRHYTQFWNYLERCRYCNVLCGEREEEARACHATHNRACRCRTGFFAHAGFCLEHASCPPGAGVIAPGTPSQNTQCQPCPPGTFSASSSSSEQCQPHRNCTALGLALNVPGSSSHDTLCTSCTGFPLSTRVPGAEECERAVIDFVAFQDISIKRLQRLLQALEAPEGWGPTPRAGRAALQLKLRRRLTELLGAQDGALLVRLLQALRVARMPGLERSVRERFLPVH

• Full Name: tumor necrosis factor receptor superfamily, member 6b, decoy
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: NM_003823.3
• Gene Symbol: TNFRSF6B
• Gene ID (NCBI): 8771
• RRID: AB_3745781
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O95407
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The tumor necrosis factor receptor super-family member 6b (TNFRSF6B) is over-expressed in various human cancers. TNFRSF6B mRNA and protein expression in the tumor tissues negatively correlated with apoptosis. Cell proliferation was decreased, cell cycle was arrested in G1/S-phase, apoptosis was increased, and migration ability was inhibited by anti-TNFRSF6B McAb in vitro. Anti-TNFRSF6B McAb could be useful to suppress proliferation and induce apoptosis in HCC. Thus, TNFRSF6B might be a critical, targeted therapy strategy for HCC (PMID: 20591579).