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Phospho-STK11/LKB1 (Ser334) Recombinant monoclonal antibody (87893-2-RR)

Phospho-STK11/LKB1 (Ser334) Recombinant monoclonal antibody

Phospho-STK11/LKB1 (Ser334) Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87893-2-RR

Clone No.252441A6

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Phospho-LKB1, Phospho-LKB1 (Ser334), Phospho-STK11, Phospho-STK11 (Ser334), Phospho-STK11/LKB1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

- +

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WB analysis using 87893-2-RR
Non-treated HeLa cells, phosphatase inhibitor treated HeLa cells and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 87893-2-RR (Phospho-STK11/LKB1 (Ser334) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (HRP-81640-5) antibody as a loading control.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in

HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87893-2-RR targets Phospho-STK11/LKB1 (Ser334) in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	serine/threonine kinase 11
Calculated Molecular Weight	49 kDa
Observed Molecular Weight	50-55 kDa
GenBank Accession Number	BC007981
Gene Symbol	LKB1
Gene ID (NCBI)	6794
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q15831
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STK11(serine/threonine-protein kinase 11) is also named as LKB1, PJS, and belongs to the protein kinase superfamily. Phospho-STK11 (Ser334), also called p-LKB1 (S334), is an inhibitory phosphorylation modification of the tumor suppressor kinase LKB1. This site is phosphorylated by Akt and PIM kinases upon growth factor stimulation. Phosphorylated Ser334 recruits 14-3-3 proteins, sequestering LKB1 in the nucleus and disrupting its binding to STRADα-MO25 complex. This abolishes LKB1's capacity to activate AMPK, impairing energy sensing, autophagy and anti-proliferative signals. Elevated p-LKB1 (Ser334) accelerates proliferation in breast, prostate and lung cancers, weakens metabolic stress response, and correlates with poor tumor prognosis. It serves as a biomarker for aberrant PI3K/Akt oncogenic signaling and represents a target to restore LKB1 tumor-suppressive function. (PMID: 22611470, 34193159)