Phospho-STK11/LKB1 (Ser334) Recombinant monoclonal antibody, PBS Only

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• Phospho-STK11/LKB1 (Ser334) Recombinant monoclonal antibody, PBS Only (87893-2-PBS)

Phospho-STK11/LKB1 (Ser334) Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 87893-2-PBS

Clone No.252441A6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

Phospho-LKB1, Phospho-LKB1 (Ser334), Phospho-STK11, Phospho-STK11 (Ser334), Phospho-STK11/LKB1

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  WB analysis using 87893-2-RR (same clone as 87893-2-PBS)

  Non-treated HeLa cells, phosphatase inhibitor treated HeLa cells and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 87893-2-RR (Phospho-STK11/LKB1 (Ser334) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (HRP-81640-5) antibody as a loading control. This data was developed using the same antibody clone with 87893-2-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

87893-2-PBS targets Phospho-STK11/LKB1 (Ser334) in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: serine/threonine kinase 11
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC007981
• Gene Symbol: LKB1
• Gene ID (NCBI): 6794
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q15831
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

STK11(serine/threonine-protein kinase 11) is also named as LKB1, PJS, and belongs to the protein kinase superfamily. Phospho-STK11 (Ser334), also called p-LKB1 (S334), is an inhibitory phosphorylation modification of the tumor suppressor kinase LKB1. This site is phosphorylated by Akt and PIM kinases upon growth factor stimulation. Phosphorylated Ser334 recruits 14-3-3 proteins, sequestering LKB1 in the nucleus and disrupting its binding to STRADα-MO25 complex. This abolishes LKB1's capacity to activate AMPK, impairing energy sensing, autophagy and anti-proliferative signals. Elevated p-LKB1 (Ser334) accelerates proliferation in breast, prostate and lung cancers, weakens metabolic stress response, and correlates with poor tumor prognosis. It serves as a biomarker for aberrant PI3K/Akt oncogenic signaling and represents a target to restore LKB1 tumor-suppressive function. (PMID: 22611470, 34193159)