Home
Products
Primary Antibodies
Recombinant Antibodies
ACTN4 Recombinant monoclonal antibody (87922-1-RR)

ACTN4 Recombinant monoclonal antibody

ACTN4 Uni-rAb^® Recombinant Antibody for WB, IHC, IP, ELISA

Cat No. 87922-1-RR

Clone No.260022G12

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

Alpha actinin 4, ACTININ 4, Actinin alpha 4, actinin, alpha 4, ACTN 4

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Filter: AllWBIPIHCAffinity

WB analysis using 87922-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 87922-1-RR (ACTN4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of HeLa using 87922-1-RR
IP result of anti-ACTN4 (IP:87922-1-RR, 4ug; Detection:87922-1-RR 1:3000) with HeLa cells lysate 960 ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 87922-1-RR
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 87922-1-RR (ACTN4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Affinity of 87922-1-RR
Biolayer interferometry (BLI) kinetic assays of 87922-1-RR against Human ACTN4 were performed. The affinity constant is 3.69 nM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, A549 cells, HepG2 cells, LNCaP cells, Raji cells, NIH/3T3 cells, rat skeletal muscle tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87922-1-RR targets ACTN4 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag13546 Product name: Recombinant human ACTN4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 612-911 aa of BC005033 Sequence: TTVTPQIINSKWEKVQQLVPKRDHALLEEQSKQQSNEHLRRQFASQANVVGPWIQTKMEEIGRISIEMNGTLEDQLSHLKQYERSIVDYKPNLDLLEQQHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMQEFRASFNHFDKDHGGALGPEEFKACLISLGYDVENDRQGEAEFNRIMSLVDPNHSGLVTFQAFIDFMSRETTDTDTADQVIASFKVLAGDKNFITAEELRRELPPDQAEYCIARMAPYQGPDAVPGALDYKSFSTALYGESDL Predict reactive species
Full Name	actinin, alpha 4
Calculated Molecular Weight	105 kDa
Observed Molecular Weight	100-105 kDa
GenBank Accession Number	BC005033
Gene Symbol	ACTN4
Gene ID (NCBI)	81
RRID	AB_3745829
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	O43707
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACTN4 (alpha actinin 4) is a ubiquitous actin-binding protein that cross-links actin filaments into bundles to form filopodia, and has a crucial role in cytoskeletal organization. ACTN4 is colocalized with actin stress fibers and is dispersed in the cytoplasm and in the nucleus. Increased levels of ACTN4 have been associated with cancer metastasis, indicating its implication in cancer cell migration and metastasis. Defects in ACTN4 cause glomerular disease. This antibody recognizes 100-105 kDa of endogenous ACTN4 protein in various lysates. (22370640, 10700177)

Protocols

Product Specific Protocols
IHC protocol for ACTN4 antibody 87922-1-RR	Download protocol
IP protocol for ACTN4 antibody 87922-1-RR	Download protocol
WB protocol for ACTN4 antibody 87922-1-RR	Download protocol