ACTN4 Recombinant monoclonal antibody, PBS Only

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• ACTN4 Recombinant monoclonal antibody, PBS Only (87922-1-PBS)

ACTN4 Uni-rAb^® Recombinant Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 87922-1-PBS

Clone No.260022G12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Alpha actinin 4, ACTININ 4, Actinin alpha 4, actinin, alpha 4, ACTN 4

• 

  WB analysis using 87922-1-RR (same clone as 87922-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87922-1-RR (ACTN4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87922-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 87922-1-RR (same clone as 87922-1-PBS)

  IP result of anti-ACTN4 (IP:87922-1-RR, 4ug; Detection:87922-1-RR 1:3000) with HeLa cells lysate 960 ug. This data was developed using the same antibody clone with 87922-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 87922-1-RR (same clone as 87922-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 87922-1-RR (ACTN4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87922-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity of 87922-1-RR

  Biolayer interferometry (BLI) kinetic assays of 87922-1-RR against Human ACTN4 were performed. The affinity constant is 3.69 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

87922-1-PBS targets ACTN4 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag13546

  Product name: Recombinant human ACTN4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 612-911 aa of BC005033

  Sequence: TTVTPQIINSKWEKVQQLVPKRDHALLEEQSKQQSNEHLRRQFASQANVVGPWIQTKMEEIGRISIEMNGTLEDQLSHLKQYERSIVDYKPNLDLLEQQHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMQEFRASFNHFDKDHGGALGPEEFKACLISLGYDVENDRQGEAEFNRIMSLVDPNHSGLVTFQAFIDFMSRETTDTDTADQVIASFKVLAGDKNFITAEELRRELPPDQAEYCIARMAPYQGPDAVPGALDYKSFSTALYGESDL

• Full Name: actinin, alpha 4
• Calculated Molecular Weight: 105 kDa
• Observed Molecular Weight: 100-105 kDa
• GenBank Accession Number: BC005033
• Gene Symbol: ACTN4
• Gene ID (NCBI): 81
• RRID: AB_3745829
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O43707
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ACTN4 (alpha actinin 4) is a ubiquitous actin-binding protein that cross-links actin filaments into bundles to form filopodia, and has a crucial role in cytoskeletal organization. ACTN4 is colocalized with actin stress fibers and is dispersed in the cytoplasm and in the nucleus. Increased levels of ACTN4 have been associated with cancer metastasis, indicating its implication in cancer cell migration and metastasis. Defects in ACTN4 cause glomerular disease. This antibody recognizes 100-105 kDa of endogenous ACTN4 protein in various lysates. (22370640, 10700177)