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Cofilin Recombinant monoclonal antibody (88053-1-RR)

Cofilin Recombinant monoclonal antibody

Cofilin Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 88053-1-RR

Clone No.260317G7

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CFL1, 18 kDa phosphoprotein, Cofilin 1, Cofilin, non-muscle isoform, Cofilin1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

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Filter: AllWBIHC

WB analysis using 88053-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 88053-1-RR (Cofilin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human appendix using 88053-1-RR
Immunohistochemical analysis of paraffin-embedded human appendix tissue slide using 88053-1-RR (Cofilin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
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MSDS SDS

Tested Applications

Positive WB detected in	A431 cells, HeLa cells, LNCaP cells, Jurkat cells, HEK-293 cells, Neuro-2a cells, PC-12 cells
Positive IHC detected in	human appendix tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

88053-1-RR targets Cofilin in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag1399 Product name: Recombinant human Cofilin protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-166 aa of BC012318 Sequence: MASGVAVSDGVIKVFNDMKVRKSSTPEEVKKRKKAVLFCLSEDKKNIILEEGKEILVGDVGQTVDDPYATFVKMLPDKDCRYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIYASSKDAIKKKLTGIKHELQANCYEEVKDRCTLAEKLGGSAVISLEGKPL Predict reactive species
Full Name	cofilin 1 (non-muscle)
Calculated Molecular Weight	18 kDa
Observed Molecular Weight	18 kDa
GenBank Accession Number	BC012318
Gene Symbol	Cofilin
Gene ID (NCBI)	1072
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P23528
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cofilin is a ubiquitous actin-binding protein required for the reorganization of actin filaments. It is a member of ADF (actin-depolymerizing factor)/cofilin family that is a key regulator of actin dynamics and essential for cellular motility, cytokinesis, and endocytosis. Cofilin activity is tightly regulated by phosphorylation and dephosphorylation.Phosphorylation at Ser3 can inhibit its activity, also causing translocation from the nucleus to the cytoplasm.