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PLEKHA1 Recombinant monoclonal antibody (88114-1-RR)

PLEKHA1 Recombinant monoclonal antibody

PLEKHA1 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 88114-1-RR

Clone No.260319B3

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

PH domain-containing family A member 1, Pleckstrin homology domain-containing family A member 1, Tandem PH domain-containing protein 1, TAPP 1, TAPP1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

- +

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WB analysis using 88114-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 88114-1-RR (PLEKHA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in

HeLa cells, A549 cells, Jurkat cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

88114-1-RR targets PLEKHA1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag0318 Product name: Recombinant human PLEKHA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-193 aa of BC001136 Sequence: MPYVDRQNRICGFLDIEENENSGKFLRRYFILDTREDSFVWYMDNPQNLPSGSSRVGAIKLTYISKVSDATKLRPKAEFCFVMNAGMRKYFLQANDQQDLVEWVNVLNKAIKITVPKQSDSQPNSDNLSRHGECGKKQVSYRTDIVGGVPIITPTQKEEVNECGESIDRNNLKRSQSHLPYFTPKPPQDSAVI Predict reactive species
Full Name	pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 1
Calculated Molecular Weight	46 kDa
Observed Molecular Weight	46 kDa
GenBank Accession Number	BC001136
Gene Symbol	PLEKHA1
Gene ID (NCBI)	59338
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9HB21
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pleckstrin homology (PH) domain is commonly found in eukaryotic signaling proteins and possesses multiple functions including the abilities to bind inositol phosphates and various proteins. The tandem PH domain containing protein-1 (TAPP1) or PH domain containing-family A (phosphoinositide binding specific) member 1 (PLEKHA1), interacts strongly and specifically with phosphatidylinositol 3,4-trisphosphate [PtdIns(3,4)P(2)], which is one of the immediate breakdown products of PtdIns(3,4,5) P (3) and functions as a signalling molecule in insulin- and growth-factor-stimulated pathways. TAPP1 is also associated with the protein-tyrosine-phosphatase-like protein-1 (PTPL1 also known as FAP-1) and maintains PTPL1 in cytoplasm. By binding to PtdIns(3,4) P (2) and PTPL1, TAPP1 may regulate the membrane localization of PTPL1.